Mechanism of inactivation of transforming deoxyribonucleic acid by X rays.

Journal of bacteriology ·Vol. 106 ·No. 1 ·1971-04-00 ·Pages 221-6

Randolph ML, Setlow JK

Abstract

Transforming deoxyribonucleic acid (DNA) from Haemophilus influenzae was exposed to X rays either in phosphate buffer or in 10% yeast extract. Relations between determinations of biological inactivation, DNA uptake by competent H. influenzae, integration of DNA into the competent cell genome, and induced single-and double-strand breaks indicate that transforming DNA is inactivated by the direct and the indirect effect of X radiation primarily because integration of DNA is prevented as a result of the production of double-strand breaks.

MeSH Terms

Buffers Carbon Isotopes Centrifugation, Density Gradient Chromosomes, Bacterial Culture Media DNA Replication DNA, Bacterial/metabolism,radiation effects Haemophilus influenzae/growth & development,metabolism,radiation effects Molecular Weight Phosphates Radiation Effects Saccharomyces Sucrose Thymidine/metabolism Transformation, Genetic Tritium

Chemicals

Buffers Carbon Isotopes Culture Media DNA, Bacterial Phosphates Tritium Sucrose Thymidine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Randolph M L

Setlow J K

References (13)

13 references, click to expand

Repair of deoxyribonucleic acid in Haemophilus influenzae. I. X-ray sensitivity of ultraviolet-sensitive mutants and their behavior as hosts to ultraviolet-irradiated bacteriophage and transforming deoxyribonucleic acid.
J Bacteriol. 1968 Feb;95(2):546-58 PMID: 5300300
Integration of deoxyribonuclease-treated DNA in bacillus subtilis transformation.
J Gen Physiol. 1966 Jul;49(6):233-58 PMID: 4961186
Physicochemical studies on x-ray inactivation of bacteriophage.
Virology. 1968 Dec;36(4):613-9 PMID: 5723671
Mapping of deletions and substitutions in heteroduplex DNA molecules of bacteriophage lambda by electron microscopy.
Science. 1969 Mar 21;163(3873):1343-8 PMID: 5765116
Ultraviolet-induced decrease in integration of Haemophilus influenzae transforming deoxyribonucleic acid in sensitive and resistant cells.
J Bacteriol. 1970 Feb;101(2):444-8 PMID: 5308769
Fate of recipient deoxyribonucleic acid during transformation in Haemophilus influenzae.
J Bacteriol. 1968 Nov;96(5):1718-24 PMID: 5303721
DNA strand breakage by x-irradiation.
Radiat Res. 1966 Nov;29(3):329-38 PMID: 4958871
SEDIMENTATION STUDIES OF THE SIZE AND SHAPE OF DNA.
J Mol Biol. 1965 Feb;11:373-90 PMID: 14290352
Repair of DNA in Haemophilus influenzae. II. Excision, repair of single-strand breaks, defects in transformation, and host cell modification in UV-sensitive mutants.
Cold Spring Harb Symp Quant Biol. 1968;33:209-18 PMID: 5305878
Ionizing radiation and ultrasonic evidence for a minimum unit of transforming principle DNA.
Biochim Biophys Acta. 1957 Nov;26(2):241-51 PMID: 13499358
Genetic transformation. II. The significance of damage to the DNA molecule.
Biochim Biophys Acta. 1959 Jun;33(2):371-87 PMID: 13670908
Development of competence of Haemophilus influenzae.
J Bacteriol. 1965 Oct;90(4):911-20 PMID: 5294817
Radiation sensitivity of bacteriophage DNA. II. Breaks and cross-links after irradiation in vivo.
Int J Radiat Biol Relat Stud Phys Chem Med. 1970;17(3):205-15 PMID: 5310377

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.106.1.221-226.1971

Published

1971-04-00

Pages

221-6

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC248665

Subset

External Links

PubMed source DOI: 10.1128/jb.106.1.221-226.1971 PMC Full Text View journal details

Analysis Services

Mechanism of inactivation of transforming deoxyribonucleic acid by X rays.

Contact

WeChat Official Account