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PMID: 53293 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Role of carbohydrate in determining the immunochemical properties of the major glycoprotein (gp71) of Friend murine leukemia virus.

Journal of virology ·Vol. 16 ·No. 6 ·1975-12-00 ·Pages 1453-63

Bolognesi DP, Collins JJ, Leis JP, Moennig V, Schäfer W, Atkinson PH

Abstract

Treatment of Friend leukemia virus gp71 with protease-free glycosidase enzymes results in removal of the major portion of the carbohydrate without affecting the amount of protein present. The digested material migrates as protein of about 60,000 to 65,000 molecular weight on sodium dodecyl sulfatepolyacrylamide gel electrophoresis. Analyses of the serological properties of gp71 after enzyme treatment indicated that the type, group, and interspecies determinants were not destroyed. In contrast, treatment with proteolytic enzymes led to the complete destruction of the gp71 molecule, including the total elimination of its serological reactivity as measured by direct and competition radioimmunoassay and by a serum cytotoxicity assay. We conclude that the carbohydrate portion of gp71 is not of major significance in defining the antigenic determinants of this viral glycoprotein.

MeSH Terms
Carbohydrates/immunology Cytotoxicity Tests, Immunologic Epitopes Friend murine leukemia virus/analysis,immunology Glycoproteins/analysis,immunology Glycoside Hydrolases/metabolism Molecular Weight Neuraminidase/metabolism Pronase/metabolism Radioimmunoassay Viral Proteins/analysis,immunology
Chemicals
Carbohydrates Epitopes Glycoproteins Viral Proteins Glycoside Hydrolases Neuraminidase Pronase
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Bolognesi D P
Collins J J
Leis J P
Moennig V
Schäfer W
Atkinson P H
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35 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.16.6.1453-1463.1975
Published
1975-12-00
Pages
1453-63
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC355754
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.16.6.1453-1463.1975 PMC Full Text View journal details
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