Rapid screening procedure for detection of plasmids in streptococci.

Journal of bacteriology ·Vol. 140 ·No. 3 ·1979-12-00 ·Pages 1112-5

Leblanc DJ, Lee LN

Abstract

An enrichment procedure, yielding plasmid deoxyribonucleic acid preparations normally containing less than 5% chromosomal contamination, has been devised for the isolation of plasmids from virtually all species of streptococci. The procedure is rapid, reproducible, and inexpensive, requiring no radioisotopes or density gradient centrifugation. The procedure can be used for routine screening of several hundred isolates in a short period of time, and plasmids obtained from 10- to 20-ml cultures can readily be visualized in agarose gels.

MeSH Terms

DNA, Bacterial/analysis DNA, Circular/analysis Dental Plaque/microbiology Humans Plasmids Species Specificity Streptococcus/analysis,genetics

Chemicals

DNA, Bacterial DNA, Circular

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Leblanc D J

Lee L N

References (15)

15 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.140.3.1112-1115.1979

Published

1979-12-00

Pages

1112-5

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC216761

Subset

External Links

PubMed source DOI: 10.1128/jb.140.3.1112-1115.1979 PMC Full Text View journal details

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Rapid screening procedure for detection of plasmids in streptococci.

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