The eighth component of human complement (C8): isolation, characterization, and hemolytic efficiency.

The Journal of experimental medicine ·Vol. 130 ·No. 5 ·1969-11-01 ·Pages 1145-60

Manni JA, Müller-Eberhard HJ

Abstract

A method was described allowing the isolation of the eighth component of complement, C8, from human serum. C8 is an immunochemically definable, heretofore unrecognized serum protein with a molecular weight of approximately 150,000 and an electrophoretic mobility of a gamma(1)-globulin. It may be obtained with 5% yield as a 5000-fold purified, physicochemically and immunochemically homogeneous protein. Under optimal experimental conditions, a few molecules of C8 were found sufficient for lysis of a cell.

MeSH Terms

Animals Blood Protein Electrophoresis Chromatography Chromatography, Gel Complement System Proteins/isolation & purification Hemolysis Humans Immunochemistry Immunoelectrophoresis Methods Rabbits Ultracentrifugation

Chemicals

Complement System Proteins

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Manni J A

Müller-Eberhard H J

References (14)

14 references, click to expand

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Article Info

Journal

The Journal of experimental medicine

Abbr.

J Exp Med

ISSN

0022-1007

DOI

10.1084/jem.130.5.1145

Published

1969-11-01

Pages

1145-60

Language

English

Region

United States

NLM ID

2985109R

PMCID

PMC2180480

Subset

External Links

PubMed source DOI: 10.1084/jem.130.5.1145 PMC Full Text View journal details

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The eighth component of human complement (C8): isolation, characterization, and hemolytic efficiency.

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