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PMID: 5409944 Published · ppublish English Journal Article

Studies on the immune response to a characterized antigenic determinant of the tobacco mosaic virus protein.

The Journal of experimental medicine ·Vol. 131 ·No. 1 ·1970-01-01 ·Pages 133-48

Spitler L, Benjamini E, Young JD, Kaplan H, Fudenberg HH

Abstract

THE FOLLOWING PEPTIDES HAVE PREVIOUSLY BEEN SHOWN TO BIND SPECIFICALLY WITH ANTIBODIES TO TMVP: (a) An eicosapeptide representing residues 93-112 of TMVP and having the sequence Ileu-Ileu-Glu-Val-Glu-AspNH(2)-GluNH(2)-Ala-AspNH(2)-Pro-Thr-Thr-Ala-Glu-Thr-Leu-Asp-Ala-Thr-Arg. (b) Its C-terminal decapeptide. (c) Its C-terminal pentapeptide. (d) N-octanoyl-C-terminal-tripeptide. (e) (Lys)(4)-C-terminal-pentapeptide. (f) (Lys)(7) C-terminal-pentapeptide. The present communication deals with the investigation of several parameters of the immunological activity of the peptides. The results show that none of the peptides tested were immunogenic in guinea pigs, nor did they stimulate the incorporation of (14)C-thymidine by spleen cells derived from TMVP-primed animals. Results also showed that all of the peptides tested could elicit specific delayed and immediate skin reactions in TMVP-sensitized guinea pigs, and furthermore, that the peptides could specifically inhibit the migration of peritoneal exudate cells derived from these animals. The elicitation of delayed skin reactions and the ability to inhibit migration of peritoneal exudate cells were independent of carrier specificity.

MeSH Terms
Animals Antibody Formation Antigens Binding Sites Carbon Isotopes Cell Movement DNA/biosynthesis Guinea Pigs Lymphocytes/immunology Peptides/analysis,pharmacology Skin/immunology Spleen/immunology Tobacco Mosaic Virus/immunology Viral Proteins/analysis
Chemicals
Antigens Carbon Isotopes Peptides Viral Proteins DNA
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Spitler L
Benjamini E
Young J D
Kaplan H
Fudenberg H H
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31 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.131.1.133
Published
1970-01-01
Pages
133-48
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2138757
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.131.1.133 PMC Full Text View journal details
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