Induction of rabbit antibody with molecular uniformity after immunization with group C streptococci.
Antibodies with uniform properties may occur in rabbits after immunization with Group C streptococci. These precipitating antibodies possess specificity for the group-specific carbohydrate. Not uncommonly, their concentration is between 20 and 40 mg/ml of antiserum. Evidence for molecular uniformity in the case of one of these antibodies, described in detail here, includes: individual antigenic specificity; monodisperse distribution of the light chains by alkaline urea polyacrylamide disc electrophoresis; and a single amino acid in each of the first three N-terminal positions of the light chains. When the amino acid sequence of rabbit antibody b+ light chains (kappa type) are aligned against their human kappa counterparts, a definite homology is observed between the N-terminus of the human and the rabbit variable region.
18 references, click to expand
-
Hydrolysis of phenylthiohydantoins of amino acids.Biochem Biophys Res Commun. 1964;14:399-403 PMID: 5836532
-
Reduction of gamma-globulins.Arch Biochem Biophys. 1962 Sep;Suppl 1:174-80 PMID: 13945475
-
Electrophoretic analysis of polypeptide chains isolated from antibodies in the serum of immunized rabbits.J Immunol. 1967 Apr;98(4):710-5 PMID: 4164553
-
Electrophoretic heterogeneity of polypeptide chains of specific antibodies.Science. 1966 May 27;152(3726):1253-5 PMID: 4160819
-
Individual Antigenic Specificity of Isolated Antibodies.Science. 1963 Jun 14;140(3572):1218-9 PMID: 17837506
-
The individual antigenic specificity of antibodies to streptococcal carbohydrates.J Exp Med. 1968 Nov 1;128(5):969-89 PMID: 4176226
-
Immune cryoglobulins in rabbit streptococcal antiserum.J Immunol. 1968 Apr;100(4):814-20 PMID: 5645222
-
N-terminal sequence heterogeneity of guinea pig anti-DNP kappa chains.Immunochemistry. 1969 Sep;6(5):645-8 PMID: 4186536
-
Studies on the chemical structure of the streptococcal cell wall. II. The composition of group C cell walls and chemical basis for serologic specificity of the carbohydrate moiety.J Exp Med. 1962 Jan 1;115:49-62 PMID: 14459433
-
Light chains of rabbit immunoglobulin: assignment to the kappa class.Science. 1967 Jun 30;156(3783):1755-7 PMID: 4180574
-
The amino-terminal amino acid sequences of rabbit immunoglobulin light chains.Proc Natl Acad Sci U S A. 1966 May;55(5):1195-201 PMID: 4163205
-
Amino acid sequence restriction in rabbit antibody light chains.Proc Natl Acad Sci U S A. 1969 Jul;63(3):890-6 PMID: 5259770
-
Characteristics of streptococcal group-specific antibody isolated from hyperimmune rabbits.J Exp Med. 1966 Apr 1;123(4):599-614 PMID: 4160396
-
Studies on human antibodies. VII. Acrylamide gel electrophoresis of purified human antibodies and myeloma proteins, their heavy and light chains.J Immunol. 1969 Feb;102(2):273-82 PMID: 5765033
-
Protein measurement with the Folin phenol reagent.J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
-
Streptococcal group-specific antibodies: occurrence of a restricted population following secondary immunization.Proc Natl Acad Sci U S A. 1968 May;60(1):134-9 PMID: 5241515
-
CHARACTERIZATION OF LAMPREY FIBRINOPEPTIDES.Biochem J. 1965 Mar;94:742-50 PMID: 14340066
-
Rabbit antibodies to streptococcal carbohydrates. Influence of primary and secondary immunization and of possible genetic factors on the antibody response.J Exp Med. 1969 Apr 1;129(4):809-30 PMID: 5766948
Contact
No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong
Qilu Normal University · Genelibs Bioinformatics Lab
750 Shunhua Rd, Jinan
2F, Bldg F, University Science Park
Tel: 0531-88819269
WeChat Official Account
Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.
Business Email
E-mail: [email protected]
Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2
