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PMID: 5463265 Published · ppublish English Journal Article

Effect of phenylalanine on protein synthesis in the developing rat brain.

The Biochemical journal ·Vol. 117 ·No. 2 ·1970-04-00 ·Pages 325-31

Agrawal HC, Bone AH, Davison AN

Abstract

1. Inhibition of the rate of incorporation of [(35)S]methionine into protein by phenylalanine was more effective in 18-day-old than in 8-day-old or adult rat brain. 2. Among the subcellular fractions incorporation of [(35)S]methionine into myelin proteins was most inhibited in 18-day-old rat brain. 3. Transport of [(35)S]methionine and [(14)C]leucine into the brain acid-soluble pool was significantly decreased in 18-day-old rats by phenylalanine (2mg/g body wt.). The decrease of the two amino acids in the acid-soluble pool equalled the inhibition of their rate of incorporation into the protein. 4. Under identical conditions, entry of [(14)C]glycine into the brain acid-soluble pool and incorporation into protein and uptake of [(14)C]acetate into lipid was not affected by phenylalanine. 5. It is proposed that decreased myelin synthesis seen in hyperphenylalaninaemia or phenylketonuria may be due to alteration of the free amino acid pool in the brain during the vulnerable period of brain development. Amyelination may be one of many causes of mental retardation seen in phenylketonuria.

MeSH Terms
Acetates/metabolism Age Factors Animals Brain/drug effects,growth & development,metabolism Carbon Isotopes Female Glycine/metabolism Humans Intellectual Disability/etiology Leucine/metabolism Male Methionine/metabolism Myelin Sheath/metabolism Phenylalanine/blood,pharmacology Phenylketonurias/complications,metabolism Protein Biosynthesis Rats Sulfur Isotopes
Chemicals
Acetates Carbon Isotopes Sulfur Isotopes Phenylalanine Methionine Leucine Glycine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Agrawal H C
Bone A H
Davison A N
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31 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1170325
Published
1970-04-00
Pages
325-31
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1178865
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1170325 PMC Full Text View journal details
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