Studies on nuclear structure and function in Tetrahymena pyriformis. 3. Comparison of the histones of macro- and micronuclei by quantitative polyacrylamide gel electrophoresis.

The Journal of cell biology ·Vol. 47 ·No. 3 ·1970-12-00 ·Pages 631-6

Gorovsky MA

Abstract

Histones were extracted from isolated macro- and micronuclear fractions and from nucleohistone fibers which were prepared from the isolated macronuclear fraction. Analysis of these histones by polyacrylamide gel electrophoresis indicated that there are electrophoretic differences between the histones of macro- and micronuclei.

MeSH Terms

Acrylates Amides Animals Cell Nucleus/analysis,physiology Densitometry

Chemicals

Acrylates Amides

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Gorovsky M A

References (13)

13 references, click to expand

Histone-bound RNA, a component of native nucleohistone.
Proc Natl Acad Sci U S A. 1965 Sep;54(3):960-7 PMID: 5217473
The electrophoresis of histones in polyacrylamide gel and their quantitative determination.
Biochem J. 1967 Jul;104(1):78-82 PMID: 6035526
Quantitative distribution of histone components in the pea plant.
Biochemistry. 1968 Feb;7(2):575-85 PMID: 5644129
Changing patterns of histone acetylation and RNA synthesis in regeneration of the liver.
Proc Natl Acad Sci U S A. 1968 Apr;59(4):1337-44 PMID: 5240033
The heterogeneity of histones. I. A quantitative analysis of calf histones in very long polyacrylamide gels.
Biochemistry. 1969 Oct;8(10):3972-9 PMID: 5346380
Simple method for quantitive densitometry of polyacrylamide gels using fast green.
Anal Biochem. 1970 Jun;35(2):359-70 PMID: 4194859
Studies on nuclear structure and function in Tetrahymena pyriformis. II. Isolation of macro- and micronuclei.
J Cell Biol. 1970 Dec;47(3):619-30 PMID: 5497543
Cell specificity of histones.
Nature. 1950 Nov 4;166(4227):780-1 PMID: 14780242
Disk electrophoresis of basic proteins and peptides on polyacrylamide gels.
Nature. 1962 Jul 21;195:281-3 PMID: 14491328
BASIC AND OTHER PROTEINS IN MICROSOMES OF RAT LIVER.
Biochem J. 1963 Aug;88:206-12 PMID: 14063858
PHYSICAL AND BIOLOGICAL PROPERTIES OF SOLUBLE NUCLEOHISTONES.
J Mol Biol. 1964 Jan;8:54-64 PMID: 14149964
ACETYLATION AND METHYLATION OF HISTONES AND THEIR POSSIBLE ROLE IN THE REGULATION OF RNA SYNTHESIS.
Proc Natl Acad Sci U S A. 1964 May;51:786-94 PMID: 14172992
THE CHROMOSOMAL SITE SPECIFYING A RIBOSOMAL PROTEIN IN ESCHERICHIA COLI.
Proc Natl Acad Sci U S A. 1964 Dec;52:1367-74 PMID: 14243510

Article Info

Journal

The Journal of cell biology

Abbr.

J Cell Biol

ISSN

0021-9525

DOI

10.1083/jcb.47.3.631

Published

1970-12-00

Pages

631-6

Language

English

Region

United States

NLM ID

0375356

PMCID

PMC2108167

Subset

External Links

PubMed source DOI: 10.1083/jcb.47.3.631 PMC Full Text View journal details

Analysis Services

Studies on nuclear structure and function in Tetrahymena pyriformis. 3. Comparison of the histones of macro- and micronuclei by quantitative polyacrylamide gel electrophoresis.

Contact

WeChat Official Account