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PMID: 5528414 Published · ppublish English Journal Article

In vitro-initiated secondary anti-hapten response. II. Increasing cell avidity for antigen.

The Journal of experimental medicine ·Vol. 132 ·No. 5 ·1970-11-00 ·Pages 926-40

Bullock WW, Rittenberg MB

Abstract

Decreasing antigen in vivo may preferentially stimulate cells with the potential for synthesis of high-affinity antibody through activation of surface receptors with similarly high affinity. This selection should result in cells with increased sensitivity to lower antigen concentrations, cells with greater avidity for antigen. We have followed the in vivo changes in anti-TNP memory-cell sensitivity by initiating the secondary anti-hapten response in vitro. This response was determined by anti-TNP plaque assay. The results indicate that cell populations with increased sensitivity for antigen continue to emerge with time after priming and that this sensitivity may increase 1000-fold in a 4 month period. Increased sensitivity to stimulation by antigen is concomitant with suppression by higher, previously stimulatory doses as in high zone immune tolerance. The data support the hypothesis that memory cells of high avidity result from the selective pressure of diminishing in vivo antigen concentration.

MeSH Terms
Amino Acids/metabolism Animals Antibodies/analysis Antibody Formation Antibody-Producing Cells Carbon Isotopes Culture Media Female Haptens Hemocyanins Immunoelectrophoresis In Vitro Techniques Injections, Intraperitoneal Mice Picrates Spleen/cytology Time Factors
Chemicals
Amino Acids Antibodies Carbon Isotopes Culture Media Haptens Picrates Hemocyanins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bullock W W
Rittenberg M B
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41 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.132.5.926
Published
1970-11-00
Pages
926-40
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2138869
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.132.5.926 PMC Full Text View journal details
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