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PMID: 561952 Published · ppublish English Journal Article

The structure of the chromatin core particle in solution.

Nucleic acids research ·Vol. 4 ·No. 9 ·1977-09-00 ·Pages 3199-214

Pardon JF, Worcester DL, Wooley JC, Cotter RI, Lilley DM, Richards RM

Abstract

The shape and size of the nucleosomal core particle from chromatin has been examined by analysis of neutron and X-ray scattering data from dilute solutions. Calculations of scattering for many different models have been made and only one model was able to account for both the X-ray and neutron profiles. This model is an oblate structure with height about 50A and diameter 110A. The DNA is mainly confined to two annuli located at the top and bottom respectively of the core particle positioned on the outside of a compact protein core which has a height of about 40A and diameter about 73A.

MeSH Terms
Animals Cell Nucleus/ultrastructure Chickens Chromatin/ultrastructure DNA/isolation & purification Deoxyribonucleoproteins/analysis Histones/analysis Micrococcal Nuclease Nucleic Acid Conformation Protein Conformation X-Ray Diffraction
Chemicals
Chromatin Deoxyribonucleoproteins Histones DNA Micrococcal Nuclease
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Pardon J F
Worcester D L
Wooley J C
Cotter R I
Lilley D M
Richards R M
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36 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/4.9.3199
Published
1977-09-00
Pages
3199-214
Language
English
Region
England
NLM ID
0411011
PMCID
PMC342644
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/4.9.3199 PMC Full Text View journal details
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