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PMID: 5635382 Published · ppublish English Journal Article

Studies on the pathogenesis of fever. XVI. Purification and further chemical characterization of granulocytic pyrogen.

The Journal of experimental medicine ·Vol. 127 ·No. 2 ·1968-02-01 ·Pages 341-57

Kozak MS, Hahn HH, Lennarz WJ, Wood WB

Abstract

Small quantities of highly purified granulocytic pyrogen have been separated from contaminating proteins by disc electrophoresis in polyacrylamide gel. The biologically active material thus isolated was shown to be electrophoretically homogeneous at pH 9 and pH 3.8. Earlier work on the chemical properties of the pyrogen molecule has been extended to include: (a) estimation of its molecular weight by gel filtration; (b) demonstration of free sulfhydryl groups essential for its biological activity; and (c) evidence that it is not inactivated by exhaustive extraction with ethanolether or n-heptane.

MeSH Terms
Animals Ascitic Fluid/analysis Cell-Free System Electrophoresis Fever/etiology Hydrogen-Ion Concentration In Vitro Techniques Leukocytes Methods Molecular Weight Proteins/analysis Pyrogens/analysis Rabbits Sulfhydryl Compounds
Chemicals
Proteins Pyrogens Sulfhydryl Compounds
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kozak M S
Hahn H H
Lennarz W J
Wood W B
References (28)
28 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.127.2.341
Published
1968-02-01
Pages
341-57
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2138451
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.127.2.341 PMC Full Text View journal details
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