Abstract
Extracts of Chromobacterium violaceum catalyzed all of the reactions involved in synthesizing tryptophan from chorismic acid. Tryptophan auxotrophs which had lost any of these activities did not produce the characteristic purple pigment, violacein, when grown on a medium in which tryptophan was limiting. Gel filtration of extracts allowed us to estimate molecular weights for the tryptophan enzymes. All of the enzymes appeared to have molecular weights below 100,000. No enzymes were observed to occur in aggregates. The specific activities of the enzymes of the tryptophan pathway did not change when mutants were grown under conditions of limiting or excess tryptophan. The first enzyme in the pathway, anthranilate synthetase, was subject to feedback control by the end product, tryptophan. Tryptophan acted as a noncompetitive inhibitor with respect to glutamine, one of the substrates for anthranilate synthetase, and as a competitive inhibitor of the reaction when chorismate, the other substrate, was varied. The nonlinearity observed in the Lineweaver-Burk plot in the latter case suggests that there may be more than one chorismate-binding site on anthranilate synthetase.
MeSH Terms
Cell-Free System
Chromatography, Gel
Chromobacterium/analysis,metabolism
Enzyme Repression
Genetics, Microbial
Ligases/metabolism
Molecular Biology
Molecular Weight
Mutation
Tryptophan/biosynthesis
ortho-Aminobenzoates
Chemicals
ortho-Aminobenzoates
Tryptophan
Ligases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wegman J
Crawford I P
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31 references, click to expand
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