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PMID: 5685878 Published · ppublish English Journal Article

The purification and some properties of a beta-lactamase (cephalosporinase) synthesized by Enterobactercloacae.

The Biochemical journal ·Vol. 109 ·No. 3 ·1968-09-00 ·Pages 469-73

Hennessey TD, Richmond MH

Abstract

1. A beta-lactamase has been purified from a strain of Enterobacter cloacae. 2. This enzyme is about eighty times as active against cephaloridine as against benzylpenicillin or ampicillin. 3. The enzyme has a net positive charge at pH8.0 and a molecular weight of about 14000. 4. An approximate amino acid composition of the enzyme is reported.

MeSH Terms
Amino Acids/analysis Ampicillin Centrifugation Cephaloridine Chromatography, Gel Enterobacteriaceae/enzymology Molecular Weight Penicillin G Penicillinase/analysis,isolation & purification
Chemicals
Amino Acids Ampicillin Penicillinase Cephaloridine Penicillin G
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hennessey T D
Richmond M H
References (14)
14 references, click to expand
  1. Interaction of cephaloridine with penicillinase-producing gram-negative bacteria.
    Nature. 1965 Oct 16;208(5007):235-7 PMID: 5326329
  2. The purification and properties of a penicillinase whose synthesis is mediated by an R-factor in Escherichia coli.
    Biochem J. 1966 Jan;98(1):204-9 PMID: 5328167
  3. Observations on the nature, distribution, and significance of cephalosporinase.
    Lancet. 1963 Jun 29;1(7296):1399-401 PMID: 13945492
  4. The biochemistry and function of beta-lactamase (penicillinase).
    Adv Enzymol Relat Areas Mol Biol. 1966;28:237-323 PMID: 5334062
  5. Inducible beta-lactamase in Enterobacter.
    J Gen Microbiol. 1967 Nov;49(2):277-85 PMID: 5183475
  6. Penicillinase synthesis controlled by infectious R factors in Enterobacteriaceae.
    Nature. 1965 Oct 16;208(5007):239-41 PMID: 5326330
  7. Differences between pencillinases from gram-positive and gram-negative bacteria.
    Nature. 1963 Mar 9;197:976-8 PMID: 13989487
  8. [Purification and physicochemical characteristics of the penicillinase of Bacillus cereus].
    C R Hebd Seances Acad Sci. 1953 Jul 20;237(3):276-8 PMID: 13082796
  9. The oxidation of ribonuclease with performic acid.
    J Biol Chem. 1956 Apr;219(2):611-21 PMID: 13319283
  10. Micro-iodometric assay for penicillinase.
    Biochem J. 1962 May;83:236-40 PMID: 14480578
  11. Infectious drug resistance.
    Br Med Bull. 1965 Sep;21(3):254-9 PMID: 5317926
  12. The amino acid sequence of Pseudomonas fluorescens azurin.
    Biochem J. 1967 Sep;104(3):784-825 PMID: 4860639
  13. Iodometric assay of penicillinase.
    Nature. 1954 Nov 27;174(4439):1012-3 PMID: 13214059
  14. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1090469
Published
1968-09-00
Pages
469-73
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1186840
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1090469 PMC Full Text View journal details
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