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PMID: 569118 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Rat hepatocyte primary cultures. IV. Maintenance in defined medium and the role of production of plasminogen activator and other proteases.

In vitro ·Vol. 14 ·No. 10 ·1978-10-00 ·Pages 824-37

Williams GM, Bermudez E, San RH, Goldblatt PJ, Laspia MF

Abstract

Primary cultures of rat hepatocytes survived well for up to 4 days in defined medium in the presence of dexamethasone but not in its absence. The loss of viability was accompanied by a loss of ultrastructural features characteristic of hepatocytes. The cultures began producing plasminogen activator and a neutral protease after 24 hr in culture. Dexamethasone inhibited the production of both of these substances. The deterioration of the cultures appeared not to be related to plasminogen activator, but prolongation of survival by a variety of protease inhibitors suggested that the neutral protease might contribute to deterioration.

MeSH Terms
Animals Cell Survival/drug effects Cells, Cultured Culture Media Dexamethasone/pharmacology Liver Peptide Hydrolases/biosynthesis Plasminogen Activators/biosynthesis Protease Inhibitors/pharmacology Rats
Chemicals
Culture Media Protease Inhibitors Dexamethasone Peptide Hydrolases Plasminogen Activators
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Williams G M
Bermudez E
San R H
Goldblatt P J
Laspia M F
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29 references, click to expand
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Article Info
Journal
In vitro
Abbr.
In Vitro
ISSN
0073-5655
DOI
10.1007/BF02616152
Published
1978-10-00
Pages
824-37
Language
English
Region
United States
NLM ID
0063733
Subset
IM
External Links
PubMed source DOI: 10.1007/BF02616152
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