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PMID: 571522 Published · ppublish English Journal Article

Chicken ribosomal DNA: gene frequency and purification by R-loop hybridization.

Molecular biology reports ·Vol. 4 ·No. 4 ·1979-02-15 ·Pages 217-21

Lehmann C, Warnhoff M, Knochel W, Lange D, Born J, Tiedemann H

Abstract

The genes for ribosomal RNA of chicken are present in about 200--240 copies per haploid genome. The ribosomal genes were separated from the bulk of DNA by different methods. In CsCl-actinomycin D gradients the GC-rich ribosomal DNA is shifted to the lower density side of the bulk of DNA. After hybridization with iodinated ribosomal RNA under conditions where R-loops are formed ribosomal DNA has an increased density. The R-loops can be separated from the bulk of DNA in Cs2-SO4 gradients.

MeSH Terms
Animals Centrifugation, Density Gradient Chick Embryo DNA/genetics Genes Nucleic Acid Hybridization RNA, Ribosomal/genetics
Chemicals
RNA, Ribosomal DNA
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Lehmann C
Warnhoff M
Knochel W
Lange D
Born J
Tiedemann H
References (23)
23 references, click to expand
  1. A general method for isolation of high molecular weight DNA from eukaryotes.
    Nucleic Acids Res. 1976 Sep;3(9):2303-8 PMID: 987581
  2. The desoxyribonucleic acid content of animal cells and its evolutionary significance.
    J Gen Physiol. 1951 Mar 20;34(4):451-62 PMID: 14824511
  3. Mechanisms of cell differentiation: Affinity of a morphogenetic factor to DNA.
    Wilhelm Roux Arch Entwickl Mech Org. 1972 Jun;171(2):160-169 PMID: 28304572
  4. Widespread presence, in chickens, of DNA complementary to the RNA genome of avian leukosis viruses.
    Proc Natl Acad Sci U S A. 1972 Mar;69(3):576-80 PMID: 4335067
  5. The ribosomal RNA cistrons.
    Prog Nucleic Acid Res Mol Biol. 1971;11:351-89 PMID: 5004308
  6. Theory of thermal transitions in low molecular weight RNA chains.
    J Mol Biol. 1968 Nov 14;37(3):445-66 PMID: 5719218
  7. On the chromosomal distribution of DNA complementary to ribosomal and soluble RNA.
    Natl Cancer Inst Monogr. 1966 Dec;23:449-72 PMID: 5963988
  8. Reliability of the RNA-DNA filter hybridization for the detection of oncornavirus-specific DNA sequences.
    J Virol. 1974 Aug;14(2):225-30 PMID: 4367899
  9. Determination of the multiplicity of the silk fibroin gene and detection of fibroin gene-related DNA in the genome of Bombyx mori.
    J Mol Biol. 1976 Mar 5;101(3):327-48 PMID: 1255722
  10. Sedimentation rate as a measure of molecular weight of DNA.
    Biophys J. 1963 Jul;3:309-21 PMID: 14016998
  11. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.
    Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408-12 PMID: 4504350
  12. Specific gene amplification in oocytes. Oocyte nuclei contain extrachromosomal replicas of the genes for ribosomal RNA.
    Science. 1968 Apr 19;160(3825):272-80 PMID: 4867987
  13. The genes for silk fibroin in Bombyx mori.
    J Mol Biol. 1972 Oct 14;70(3):637-49 PMID: 5083150
  14. Biological activity of 125iodine labelled globin mRNA in an Ehrlich ascites cell-free system.
    Mol Biol Rep. 1974 Mar;1(6):311-20 PMID: 4427594
  15. Messenger RNA synthesis during amino acid starvation in Escherichia coli.
    J Mol Biol. 1968 Oct 28;37(2):269-88 PMID: 4939044
  16. DETERMINATION OF THE CODING RATIO BASED ON MOLECULAR WEIGHT OF MESSENGER RIBONUCLEIC ACID ASSOCIATED WITH ERGOSOMES OF DIFFERENT AGGREGATE SIZE.
    Nature. 1964 Jan 18;201:264-70 PMID: 14110449
  17. The structural organization of ribosomal DNA in Drosophila melanogaster.
    Cell. 1977 Feb;10(2):193-212 PMID: 402222
  18. Hybridization of 125I-labeled ribonucleic acid.
    Biochemistry. 1973 Nov 6;12(23):4675-9 PMID: 4589944
  19. Retention of common nucleotide sequences in the ribosomal deoxyribonucleic acid of eukaryotes and some of their physical characteristics.
    Biochemistry. 1971 Jul 6;10(14):2761-9 PMID: 5105183
  20. Isolation of high-molecular-weight DNA from mammalian cells.
    Eur J Biochem. 1973 Jul 2;36(1):32-8 PMID: 4200179
  21. Analysis of chicken ribosomal RNA genes and construction of lambda hybrids containing gene fragments.
    Science. 1977 Apr 8;196(4286):195-7 PMID: 557836
  22. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  23. R loop mapping of the 18S and 28S sequences in the long and short repeating units of Drosophila melanogaster rDNA.
    Cell. 1977 Feb;10(2):177-92 PMID: 402221
Article Info
Journal
Molecular biology reports
Abbr.
Mol Biol Rep
ISSN
0301-4851
DOI
10.1007/BF00777557
Published
1979-02-15
Pages
217-21
Language
English
Region
Netherlands
NLM ID
0403234
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00777557 View journal details
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