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PMID: 58054 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Isolation and properties of the leukocytosis- and lymphocytosis-promoting factor of Bordetella pertussis.

The Journal of experimental medicine ·Vol. 143 ·No. 6 ·1976-06-01 ·Pages 1483-502

Morse SI, Morse JH

Abstract

The leukocytosis- and lymphocytosis-promoting factor (LPF) of Bordetella pertussis has been isolated to near homogeneity by physical, chemical, and electron microscopical criteria. LPF contains 14.5% nitrogen and is lipid and carbohydrate free. It is apparently composed of four polypeptide subunits. LPF caused leukocytosis and lymphocytosis in "nude" as well as in normal mice. In addition, purified LPF also induced histamine sensitization and hypoglycemia and refractoriness to the hyperglycemic effect of epinephrine. A monospecific LPF antiserum blocked these reactions as well as leukocytosis and lymphocytosis. LPF is clearly distinct from the hemagglutinating pili of B. pertussis.

MeSH Terms
Amino Acids/analysis Animals Antigens, Bacterial/isolation & purification Bacterial Proteins/analysis,immunology,isolation & purification Bordetella pertussis/immunology Epinephrine/pharmacology Epitopes Hemagglutination Inhibition Tests Hemagglutinins/analysis,isolation & purification Histamine/pharmacology Hypoglycemia/etiology Isoelectric Point Leukocytosis/immunology Lymphocytosis/immunology Mice Neutrophils
Chemicals
Amino Acids Antigens, Bacterial Bacterial Proteins Epitopes Hemagglutinins Histamine Epinephrine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Morse S I
Morse J H
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27 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.143.6.1483
Published
1976-06-01
Pages
1483-502
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2190226
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.143.6.1483 PMC Full Text View journal details
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