Ribosomal proteins in the fungus Podospora anserina: evidence for an electrophoretically altered 60S protein in a cycloheximide resistant mutant.

Molecular & general genetics : MGG ·Vol. 156 ·No. 2 ·1977-11-14 ·Pages 141-4

Begueret J, Perrot M, Crouzet M

Abstract

Proteins of cytoplasmic ribosomes of the Podospora anserina were analyzed by two dimensional gel electrophoresis. The numbers of proteins were estimated to be 28 in the small subunit and 41 in the large subunit. The L21 protein of the large subunit was found to migrate differently in a cycloheximide resistant mutant.

MeSH Terms

Ascomycota/genetics Cycloheximide/pharmacology Drug Resistance, Microbial Electrophoresis Fungal Proteins/analysis,genetics Phenotype Protein Precursors/analysis Ribosomal Proteins/analysis,genetics Xylariales/genetics

Chemicals

Fungal Proteins Protein Precursors Ribosomal Proteins Cycloheximide

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Begueret J

Perrot M

Crouzet M

References (9)

9 references, click to expand

Study on proteins from yeast cytoplasmic ribosomes by two-dimensional gel electrophoresis.
Mol Gen Genet. 1976 Apr 23;145(1):73-9 PMID: 5666
The elongation steps in protein synthesis by eukaryotic ribosomes: effects of antibiotics.
Methods Enzymol. 1974;30:282-9 PMID: 4605255
A comparison of the proteins of rat skeletal muscle and liver ribosomes by two-dimensional polyacrylamide gel electrophoresis. Observations on the partition of proteins between ribosomal subunits and a description of two acidic proteins in the large subunit.
J Biol Chem. 1974 Apr 10;249(7):2258-67 PMID: 4594498
Ribosomal proteins from rabbit reticulocytes: number and molecular weights of proteins from ribosomal subunits.
J Mol Biol. 1975 Apr 15;93(3):391-404 PMID: 49436
The extraction of proteins from eukaryotic ribosomes and ribosomal subunits.
Mol Gen Genet. 1974;135(2):97-112 PMID: 4457758
Characterization of chloroplast and cytoplasmic ribosomal proteins of Chlamydomonas reinhardi by two-dimensional gel electrophoresis.
Mol Gen Genet. 1974;132(2):105-18 PMID: 4417561
Separation and radioautography of microgram quantities of ribosomal proteins by two-dimensional polyacrylamide gel electrophoresis.
FEBS Lett. 1973 Jan 15;29(2):177-80 PMID: 4578220
Genetic evidence of ribosomal antisuppressors in Podospora anserina.
Mol Gen Genet. 1976 Sep 23;147(3):299-306 PMID: 967160
Ribosomal proteins. VII. Two-dimensional polyacrylamide gel electrophoresis for fingerprinting of ribosomal proteins.
Anal Biochem. 1970 Aug;36(2):401-12 PMID: 4916449

Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00283486

Published

1977-11-14

Pages

141-4

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00283486

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Ribosomal proteins in the fungus Podospora anserina: evidence for an electrophoretically altered 60S protein in a cycloheximide resistant mutant.

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