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PMID: 967160 Published · ppublish English Journal Article

Genetic evidence of ribosomal antisuppressors in Podospora anserina.

Molecular & general genetics : MGG ·Vol. 147 ·No. 3 ·1976-09-23 ·Pages 299-306

Picard-Bennoun M

Abstract

Antisuppressors were screened for with the help of informational suppressors in Podospores anserina. Four mutations in the AS1 locus and two in the AS2 locus were isolated, using allele non specific suppressors supposed to be ribosomal ambiguity mutations. Four mutations in the AS3 locus and 45 in the AS4 locus were obtained, using a nonsense (t-RNA like) suppressor. All antisuppressors are partially dominant. Most mutations in the AS4 locus are lethal. The four mutants at the AS3 locus and 6 out of the 8 viable mutants at the AS4 locus are cold sensitive. Phenotypic properties and action spectra of the antisuppressors suggest that they are restrictive ribosomal mutations.

MeSH Terms
Ascomycota/metabolism Ethyl Methanesulfonate/pharmacology Genes, Dominant Mutagens Mutation Nitrosoguanidines/pharmacology Phenotype Protein Biosynthesis Ribosomes/metabolism Spores Suppression, Genetic
Chemicals
Mutagens Nitrosoguanidines Ethyl Methanesulfonate
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Picard-Bennoun M
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28 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00582881
Published
1976-09-23
Pages
299-306
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00582881
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