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PMID: 6066049 Published · ppublish English Journal Article

Phospholipids of Thiobacillus thiooxidans.

Journal of bacteriology ·Vol. 94 ·No. 5 ·1967-11-00 ·Pages 1679-83

Shively JM, Benson AA

Abstract

Cells and spent growth media from sulfur- and thiosulfate-grown cultures of Thiobacillus thiooxidans were analyzed. The phosphatides were examined by thinlayer chromatography, and the products of their hydrolysis by hydrochloric acid and methanolic potassium hydroxide were separated by paper chromatography. The phospholipids in both cells and spent growth media were identified as phosphatidyl ethanolamine, phosphatidyl-N-monomethylethanolamine, phosphatidyl glycerol, and diphosphatidyl glycerol. These comprised about 97% of the total lipid phosphorus. Lyso-phosphatidyl-N-monomethylethanolamine and lysophosphatidyl glycerol accounted for the remaining 3%. The percentage of the total lipid phosphorus accounted for by each phospholipid depended on the age of the culture.

MeSH Terms
Autoradiography Carbon Isotopes Chromatography, Paper Chromatography, Thin Layer Culture Media/analysis Hydrochloric Acid Hydroxides Phosphatidylethanolamines/analysis Phospholipids/analysis Phosphorus Isotopes Potassium Sulfur/metabolism Thiobacillus/analysis,growth & development Thiosulfates/metabolism
Chemicals
Carbon Isotopes Culture Media Hydroxides Phosphatidylethanolamines Phospholipids Phosphorus Isotopes Thiosulfates Sulfur Hydrochloric Acid Potassium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Shively J M
Benson A A
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24 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.94.5.1679-1683.1967
Published
1967-11-00
Pages
1679-83
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC276878
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.94.5.1679-1683.1967 PMC Full Text View journal details
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