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PMID: 6083561 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Protein export in Escherichia coli requires a soluble activity.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 24 ·1984-12-00 ·Pages 7737-41

Müller M, Blobel G

Abstract

By using a reconstituted cell-free system we have demonstrated the existence of a soluble activity that is required for the export of proteins in Escherichia coli. This export factor sediments at about 12 S. It has been partially purified by passage through an omega-NH2-butylagarose column and by salt elution off a DEAE matrix. The export factor does not contain 6S RNA.

MeSH Terms

Bacterial Proteins/metabolism Cell-Free System Electrophoresis, Polyacrylamide Gel Escherichia coli/metabolism Molecular Weight Protein Biosynthesis RNA, Bacterial/genetics,isolation & purification

Chemicals

Bacterial Proteins RNA, Bacterial

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Müller M

Blobel G

References (25)

25 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.81.24.7737

Published

1984-12-00

Pages

7737-41

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC392227

Subset

Grants

NIGMS NIH HHS · GM 27135 · United States

External Links

PubMed source DOI: 10.1073/pnas.81.24.7737 PMC Full Text View journal details

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Protein export in Escherichia coli requires a soluble activity.

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