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PMID: 6390437 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

In vitro translocation of bacterial proteins across the plasma membrane of Escherichia coli.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 23 ·1984-12-00 ·Pages 7421-5

Müller M, Blobel G

Abstract

Precursors to two periplasmic proteins and one outer membrane protein were synthesized in a membrane-free extract from Escherichia coli programmed with plasmid DNA. In the presence of inverted plasma membrane vesicles from E. coli up to 25% of the precursor molecules were converted into their mature forms. Using externally added proteinase K as a probe, we found the processed proteins segregated within the membrane vesicles. By the same criteria, a small amount of each precursor also proved to be translocated, indicating that translocation and signal sequence cleavage are not necessarily coupled processes. Furthermore, we present conclusive evidence that the translocation step can occur post-translationally even as late as 60 min after the beginning of translation.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics,isolation & purification,metabolism Biological Transport Cell Membrane/metabolism Electrophoresis, Polyacrylamide Gel Escherichia coli/metabolism Kinetics Plasmids Protein Biosynthesis Protein Processing, Post-Translational Transcription, Genetic
Chemicals
Bacterial Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Müller M
Blobel G
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45 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.23.7421
Published
1984-12-00
Pages
7421-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC392158
Subset
IM
Grants
NIGMS NIH HHS · GM 27155 · United States
External Links
PubMed source DOI: 10.1073/pnas.81.23.7421 PMC Full Text View journal details
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