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PMID: 6084655 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Masking of epitopes in tissue sections. A study of glial fibrillary acidic (GFA) protein with antisera and monoclonal antibodies.

Histochemistry ·Vol. 81 ·No. 6 ·1984-00-00 ·Pages 525-31

Dahl D, Grossi M, Bignami A

Abstract

Antisera to chicken brain antigen (CBA) isolated by hydroxyapatite chromatography from 8 M urea extracts following repeated extractions with phosphate buffer selectively decorate neurofilaments (NF) in neuronal perikarya, dendrites and axons. The antisera also reacted with GFA protein, the astrocyte-specific intermediate filament protein, as indicated by the adsorption of NF immunoreactivity following passage of the antisera through columns prepared with purified GFA protein. Moreover, the antisera stained the polypeptides of the NF triplet (70 kd, 150 kd, 200 kd) and GFA protein by the immunoblotting procedure. Monoclonal antibodies selectively decorating NF in tissue sections were isolated from a fusion of mouse myeloma cells with spleen cells of mice immunized with CBA. By the immunoblotting procedure the antibodies decorated the 150 kd NF polypeptide and GFA protein. No staining of glial filaments or any other structure on tissue sections was also observed with antibodies derived from another fusion strongly reacting with GFA protein on immunoblots. All antibodies (monoclonal and polyclonal) appeared to react with the same region of the GFA polypeptide as indicated by immunoblots of cleavage products.

MeSH Terms
Animals Antibodies, Monoclonal Antigen-Antibody Complex Cattle Chickens Epitopes/analysis Glial Fibrillary Acidic Protein/analysis,immunology Immune Sera Optic Nerve/analysis Purkinje Cells/cytology Spinal Cord/analysis,cytology
Chemicals
Antibodies, Monoclonal Antigen-Antibody Complex Epitopes Glial Fibrillary Acidic Protein Immune Sera
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Dahl D
Grossi M
Bignami A
References (36)
36 references, click to expand
  1. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  2. Monoclonal antibodies to intermediate filament proteins of human cells: unique and cross-reacting antibodies.
    J Cell Biol. 1982 Nov;95(2 Pt 1):414-24 PMID: 6183272
  3. Isolation of neurofilament proteins and of immunologically active neurofilament degradation products from extracts of brain, spinal cord and sciatic nerve.
    Biochim Biophys Acta. 1981 Apr 28;668(2):299-306 PMID: 7194689
  4. Neurons cultured from developing rat brain attach and spread preferentially to laminin.
    J Neurosci Res. 1984;11(3):241-51 PMID: 6737517
  5. Sequence of a cDNA clone encoding mouse glial fibrillary acidic protein: structural conservation of intermediate filaments.
    Proc Natl Acad Sci U S A. 1984 May;81(9):2743-6 PMID: 6585825
  6. Isolation of polymerization-competent vimentin from porcine eye lens tissue.
    FEBS Lett. 1981 Mar 23;125(2):253-6 PMID: 7227553
  7. Monoclonal antibodies to gel-excised glial filament protein and their reactivities with other intermediate filament proteins.
    J Neurochem. 1984 Jan;42(1):25-32 PMID: 6358415
  8. Astroglial and axonal proteins in isolated brain filaments. II. Isolation of a 70 000-dalton polypeptide from bovine brain filament preparations by immunoaffinity chromatography with antineurofilament antisera.
    Biochim Biophys Acta. 1980 Mar 26;622(1):9-17 PMID: 6767503
  9. Immunohistochemical differences between neurofilaments in perikarya, dendrites and axons. Immunofluorescence study with antisera raised to neurofilament polypeptides (200K, 150K, 70K) isolated by anion exchange chromatography.
    Exp Cell Res. 1983 Dec;149(2):397-408 PMID: 6416876
  10. Neurofilament and glial fibrillary acid protein-related immunoreactivity in rodent enteric nervous system.
    Neuroscience. 1984 May;12(1):277-87 PMID: 6379500
  11. Purification of a brain-specific astroglial protein by immunoaffinity chromatography.
    Anal Biochem. 1978 Sep;89(2):360-71 PMID: 727439
  12. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 PMID: 388439
  13. Turbidimetric studies of the in vitro assembly and disassembly of porcine neurotubules.
    J Mol Biol. 1974 Nov 15;89(4):737-55 PMID: 4475698
  14. Vimentin in the central nervous system. A study of the mesenchymal-type intermediate filament-protein in Wallerian degeneration and in postnatal rat development by two-dimensional gel electrophoresis.
    Differentiation. 1982;22(3):185-90 PMID: 7173527
  15. In vivo and in vitro differentiation of neurons and astrocytes in the rat embryo. Immunofluorescence study with neurofilament and glial filament antisera.
    Dev Biol. 1981 Jul 30;85(2):344-57 PMID: 6790318
  16. Appearance and distribution of neurofilament immunoreactivity in iris nerves.
    J Comp Neurol. 1984 Mar 1;223(3):457-70 PMID: 6423704
  17. Laminin in rat sciatic nerve undergoing Wallerian degeneration. Immunofluorescence study with laminin and neurofilament antisera.
    J Neuropathol Exp Neurol. 1984 Jan;43(1):94-103 PMID: 6693930
  18. Astroglial and axonal proteins in isolated brain filaments. I. Isolation of the glial fibrillary acidic protein and of an immunologically active cyanogen bromide peptide from brain filament preparations of bovine white matter.
    Biochim Biophys Acta. 1979 Jun 19;578(2):305-16 PMID: 39623
  19. Specific neuronal localization by immunofluorescence of 10 nm filament polypeptides.
    J Neurocytol. 1980 Dec;9(6):835-44 PMID: 7009798
  20. Related amino acid sequences in neurofilaments and non-neural intermediate filaments.
    Nature. 1982 Apr 1;296(5856):448-50 PMID: 7199625
  21. Amino acid sequence data on glial fibrillary acidic protein (GFA); implications for the subdivision of intermediate filaments into epithelial and non-epithelial members.
    EMBO J. 1983;2(11):2059-63 PMID: 6685624
  22. Antibody decoration of neurofilaments.
    J Cell Biol. 1981 May;89(2):198-205 PMID: 6788775
  23. Isolation and characterization of a soluble, immunoactive peptide of glial fibrillary acidic protein.
    Biochim Biophys Acta. 1981 Sep 29;670(2):139-45 PMID: 6794638
  24. Preparation of antisera to neurofilament protein from chicken brain and human sciatic nerve.
    J Comp Neurol. 1977 Dec 15;176(4):645-57 PMID: 72760
  25. Expression of a neural type of intermediate filament as a distinguishing feature between oat cell carcinoma and other lung cancers.
    Am J Pathol. 1983 Feb;110(2):113-8 PMID: 6297305
  26. High resolution two-dimensional electrophoresis of proteins.
    J Biol Chem. 1975 May 25;250(10):4007-21 PMID: 236308
  27. The characterization of tubulin in CNS membrane fractions.
    J Neurochem. 1981 Nov;37(5):1295-307 PMID: 7299399
  28. All classes of intermediate filaments share a common antigenic determinant defined by a monoclonal antibody.
    Cell. 1981 Dec;27(3 Pt 2):419-28 PMID: 6086105
  29. Cell-specific domains of glial- and muscle-type intermediate filament proteins. Immunoaffinity chromatography and immunoblotting study of GFA protein and desmin.
    Exp Cell Res. 1984 Oct;154(2):464-73 PMID: 6479239
  30. Neurofibrillary changes in human brain. An immunocytochemical study with a neurofilament antiserum.
    J Neuropathol Exp Neurol. 1983 Jan;42(1):69-79 PMID: 6337236
  31. The amino acid sequence of chicken muscle desmin provides a common structural model for intermediate filament proteins.
    EMBO J. 1982;1(12 ):1649-56 PMID: 6202512
  32. Neuroendocrine carcinoma of the skin (Merkel cell carcinoma): ultrastructural and immunohistochemical demonstration of neurofilaments.
    Ultrastruct Pathol. 1983 Mar-Apr;4(2-3):219-25 PMID: 6349064
  33. Filament proteins in rat optic nerves undergoing Wallerian degeneration.
    Exp Neurol. 1981 Feb;71(2):421-30 PMID: 7192639
  34. Purified glial fibrillary acidic protein and desmin are distinct intermediate filament proteins exhibiting similar properties.
    J Biol Chem. 1981 Oct 25;256(20):10606-12 PMID: 7287726
  35. Immunohistochemical localization of neurofilament antigen in rat cerebellum.
    J Neurocytol. 1979 Aug;8(4):513-25 PMID: 490194
  36. Purification of the glial fibrillary acidic protein by anion-exchange chromatography.
    Anal Biochem. 1982 Oct;126(1):165-9 PMID: 7181107
Article Info
Journal
Histochemistry
Abbr.
Histochemistry
ISSN
0301-5564
DOI
10.1007/BF00489531
Published
1984-00-00
Pages
525-31
Language
English
Region
Germany
NLM ID
0411300
Subset
IM
Grants
NINDS NIH HHS · NS 13034 · United States
External Links
PubMed source DOI: 10.1007/BF00489531
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