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PMID: 6086584 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Expression of the gene encoding protein A in Staphylococcus aureus and coagulase-negative staphylococci.

Journal of bacteriology ·Vol. 159 ·No. 2 ·1984-08-00 ·Pages 713-9

Uhlén M, Guss B, Nilsson B, Götz F, Lindberg M

Abstract

Two shuttle vectors containing the gene for protein A (spa) from Staphylococcus aureus have been constructed to study expression of the gene in various strains of S. aureus and in the coagulase-negative species Staphylococcus epidermidis, Staphylococcus capitis, and Staphylococcus xylosus. One plasmid, pSPA15, contains the complete structural gene for protein A, which binds to the cell wall in various Staphylococcus species. The other plasmid, pSPA16, codes for a truncated protein A lacking the C-terminal part called region X. The latter is exclusively extracellular in all Staphylococcus species tested, which confirms the importance of region X for cell wall binding. The expression of the plasmid-coded protein A in various strains of S. aureus is strongly correlated to the expression of the chromosomal spa gene. The coagulase-negative species expressing plasmid-encoded protein A produce 12 to 30% of the amount coded by the chromosomal spa gene in S. aureus strains Cowan I and A676.

MeSH Terms
Amino Acid Sequence Base Sequence Coagulase/genetics DNA Restriction Enzymes Genes Genes, Bacterial Plasmids Species Specificity Staphylococcal Protein A/genetics Staphylococcus/genetics Staphylococcus aureus/genetics
Chemicals
Coagulase Staphylococcal Protein A DNA Restriction Enzymes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Uhlén M
Guss B
Nilsson B
Götz F
Lindberg M
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38 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.159.2.713-719.1984
Published
1984-08-00
Pages
713-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC215703
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.159.2.713-719.1984 PMC Full Text View journal details
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