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PMID: 6087277 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Interruption of a human nuclear sequence homologous to mitochondrial DNA by a member of the KpnI 1.8 kb family.

Nucleic acids research ·Vol. 12 ·No. 13 ·1984-07-11 ·Pages 5225-34

Nomiyama H, Tsuzuki T, Wakasugi S, Fukuda M, Shimada K

Abstract

We reported that several DNA sequences homologous to mitochondrial DNA (mtDNA) are present in the human nuclear genome (Tsuzuki et al. (1983) Gene 25, 223-229). Detailed Southern blot analyses revealed that one of such sequences is interrupted by a repetitive sequence about 1.8 kb long, and that the insert is one member of the dispersed repeated DNA sequences of the KpnI 1.8 kb family. Nucleotide sequence analysis showed that the KpnI 1.8 kb DNA is flanked with imperfect 15-base pair (bp) direct repeats of mtDNA. This KpnI 1.8 kb DNA has an A-rich sequence at its 3'-end, and has a considerable homology with one of the published cDNA sequences homologous to one of the human KpnI families and also to one of the African green monkey KpnI families, KpnI-LS1. These structural features suggest that the KpnI 1.8 kb DNA is a movable element and is inserted within the mtDNA-like sequence by an RNA-mediated process.

MeSH Terms
Animals Base Composition Base Sequence Cell Nucleus/metabolism Chlorocebus aethiops DNA/analysis,genetics DNA Restriction Enzymes/metabolism DNA, Mitochondrial/genetics Deoxyribonucleases, Type II Site-Specific Escherichia coli/genetics Genes Humans Nucleic Acid Hybridization Plasmids Species Specificity
Chemicals
DNA, Mitochondrial DNA DNA Restriction Enzymes Deoxyribonucleases, Type II Site-Specific GGTACC-specific type II deoxyribonucleases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Nomiyama H
Tsuzuki T
Wakasugi S
Fukuda M
Shimada K
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27 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.13.5225
Published
1984-07-11
Pages
5225-34
Language
English
Region
England
NLM ID
0411011
PMCID
PMC318915
Subset
IM
Databases
GENBANK
X00743, X00744
External Links
PubMed source DOI: 10.1093/nar/12.13.5225 PMC Full Text View journal details
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