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PMID: 6087345 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Small fragments of herpesvirus DNA with transforming activity contain insertion sequence-like structures.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 15 ·1984-08-00 ·Pages 4736-40

Galloway DA, Nelson JA, McDougall JK

Abstract

A 737-base-pair fragment of herpes simplex virus type 2 DNA with morphological-transforming ability was identified by transfecting into rodent cells deleted fragments of the left-hand end of the Bgl II N fragment region (map position 0.58-0.625), which were constructed in vitro. The transforming sequences lie within the coding region for a Mr 61,000 protein, but the fragment itself does not appear to specify a viral polypeptide. Contained within the transforming fragment are sequences that can be drawn as a stem-loop structure flanked by direct repeats, similar to an insertion sequence-like element. An insertion sequence-like structure was also found in a small fragment of human cytomegalovirus DNA that has transforming activity. Possible mechanisms of herpesvirus transformation are discussed.

MeSH Terms
Animals Base Sequence Cell Transformation, Viral Cells, Cultured DNA Transposable Elements DNA, Viral/genetics Genes Nucleic Acid Conformation Rats Simplexvirus/genetics Transfection
Chemicals
DNA Transposable Elements DNA, Viral
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Galloway D A
Nelson J A
McDougall J K
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39 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.15.4736
Published
1984-08-00
Pages
4736-40
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC391565
Subset
IM
Grants
NCI NIH HHS · CA26001 · United States
NCI NIH HHS · CA29350 · United States
NCI NIH HHS · F32 CA06986 · United States
Databases
GENBANK
K02023, K02911, M12700
External Links
PubMed source DOI: 10.1073/pnas.81.15.4736 PMC Full Text View journal details
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