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PMID: 6090396 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Isolation of transformation-deficient Streptococcus pneumoniae mutants defective in control of competence, using insertion-duplication mutagenesis with the erythromycin resistance determinant of pAM beta 1.

Journal of bacteriology ·Vol. 159 ·No. 3 ·1984-09-00 ·Pages 870-6

Morrison DA, Trombe MC, Hayden MK, Waszak GA, Chen JD

Abstract

Several transformation-deficient mutants of Streptococcus pneumoniae were isolated after insertion-duplication mutagenesis. Mutagenesis was accomplished by transformation of competent cells with chimeric DNA formed by the ligation of TaqI fragments of pneumococcal DNA to the erythromycin resistance determinant of the streptococcal plasmid pAM beta 1. The two mutants described were characterized as defective in the control of competence induction, possibly due to a block in the production of the intercellular competence-inducing protein.

MeSH Terms
Chimera DNA Transposable Elements Drug Resistance, Microbial Erythromycin/toxicity Genetic Linkage Genotype Mutation Species Specificity Streptococcus pneumoniae/genetics Transformation, Bacterial
Chemicals
DNA Transposable Elements Erythromycin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Morrison D A
Trombe M C
Hayden M K
Waszak G A
Chen J D
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32 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.159.3.870-876.1984
Published
1984-09-00
Pages
870-6
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC215739
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.159.3.870-876.1984 PMC Full Text View journal details
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