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PMID: 6619096 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Isolation and characterization of three new classes of transformation-deficient mutants of Streptococcus pneumoniae that are defective in DNA transport and genetic recombination.

Journal of bacteriology ·Vol. 156 ·No. 1 ·1983-10-00 ·Pages 281-90

Morrison DA, Lacks SA, Guild WR, Hageman JM

Abstract

Transformation-deficient mutants of Streptococcus pneumoniae were isolated after nitrosoguanidine mutagenesis. Seventeen mutants developed normal peaks of competence, as tested by their ability to degrade one strand of donor DNA, but they yielded transformants for chromosomal point markers at efficiencies from less than 0.001 to 0.04 that of the wild type. Some of the mutants were defective in DNA uptake and are described as entry defective (Ent-). Others took up DNA in normal quantities, but they failed to give stable transformants and are described as recombination defective (Rec-). In two of the Rec- mutants, normal levels of transformation by plasmid DNA occurred; in the others, it was reduced as much as chromosomal transformation. Conjugative transfers of a chromosomal omega (cat tet) element and of the plasmid pIP501 occurred at normal levels both to and from Rec- mutants. Transfer of chloramphenicol resistance by transformation with omega (cat tet) donor DNA, however, was blocked in Rec- mutants to about the same extent as was transformation for point markers.

MeSH Terms
Conjugation, Genetic DNA, Bacterial/metabolism Genes, Bacterial Mutation Phenotype Recombination, Genetic Streptococcus pneumoniae/genetics,metabolism Transformation, Bacterial
Chemicals
DNA, Bacterial
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Morrison D A
Lacks S A
Guild W R
Hageman J M
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42 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.156.1.281-290.1983
Published
1983-10-00
Pages
281-90
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC215081
Subset
IM
Grants
NIAID NIH HHS · AI 16783 · United States
NIAID NIH HHS · AI 17841 · United States
NIGMS NIH HHS · GM 29721 · United States
External Links
PubMed source DOI: 10.1128/jb.156.1.281-290.1983 PMC Full Text View journal details
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