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PMID: 21166 Published · ppublish English Journal Article

Transformation in pneumococcus: existence and properties of a complex involving donor deoxyribonucleate single strands in eclipse.

Journal of bacteriology ·Vol. 132 ·No. 2 ·1977-11-00 ·Pages 576-83

Morrison DA

Abstract

Donor deoxyribonucleic acid (DNA) single strands exist in a complex during the eclipse phase in pneumococcal transformation. This eclipse complex exhibited specific physical properties distinct from those of both pure DNA single strands and native DNA. These included a lower affinity for diethylaminoethyl-cellulose and hydroxylapatite than that of single-strand DNA, faster sedimentation than the DNA chains that it contains, and a buoyant density in Cs2SO4 lower than that of native DNA. The complex was dissociated by treatments with sodium dodecyl sulfate, NaOH, guanidine-hydrochloride, chloroform, and proteinase K but was insensitive to ribonuclease.

MeSH Terms
Centrifugation, Isopycnic Chromatography DNA, Bacterial/analysis,genetics DNA, Single-Stranded/analysis,genetics Streptococcus pneumoniae/analysis,genetics Transformation, Bacterial
Chemicals
DNA, Bacterial DNA, Single-Stranded
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Morrison D A
References (25)
25 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.132.2.576-583.1977
Published
1977-11-00
Pages
576-83
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC221898
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.132.2.576-583.1977 PMC Full Text View journal details
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