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PMID: 6091140 Published · ppublish English Journal Article

Human T-cell leukemia virus (HTLV-I) transcripts in fresh and cultured cells of patients with adult T-cell leukemia.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 19 ·1984-10-00 ·Pages 6207-11

Franchini G, Wong-Staal F, Gallo RC

Abstract

We examined the DNA and RNA of fresh leukemic cells (five patients) and long-term cultured T lymphocytes (four patients) from patients with adult T-cell leukemia for the presence of human T-cell leukemia virus (HTLV-I) sequences. In all cases HTLV-I provirus was found and several species of viral mRNAs were observed in all cell lines. We used HTLV DNA probes representing the gag, pol, and env genes and pX regions and the U3- and U5-specific sequences to characterize the genetic content of the viral transcripts. Although similar RNA sequences were expressed in one HTLV-I provirus-positive specimen of fresh primary leukemic cells, no viral transcripts were found in four other similar specimens. These findings are consistent with the idea that the expression of one or more HTLV-I genes may be involved in initiation of transformation, but consistent expression is not needed for maintaining the neoplastic state.

MeSH Terms
Adult Cell Line Cells, Cultured DNA, Neoplasm/genetics,isolation & purification DNA, Viral/genetics,isolation & purification Deltaretrovirus/genetics Humans Leukemia/microbiology Nucleic Acid Hybridization Plasmids RNA, Neoplasm/genetics,isolation & purification RNA, Viral/genetics,isolation & purification T-Lymphocytes Transcription, Genetic
Chemicals
DNA, Neoplasm DNA, Viral RNA, Neoplasm RNA, Viral
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Franchini G
Wong-Staal F
Gallo R C
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32 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.19.6207
Published
1984-10-00
Pages
6207-11
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC391889
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.81.19.6207 PMC Full Text View journal details
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