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PMID: 6300858 Published · ppublish English Journal Article

Human T-cell leukemia-lymphoma virus (HTLV): cloning of an integrated defective provirus and flanking cellular sequences.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 6 ·1983-03-00 ·Pages 1574-8

Manzari V, Wong-Staal F, Franchini G, Colombini S, Gelmann EP, Oroszlan S, Staal S, Gallo RC

Abstract

Human T-cell leukemia-lymphoma virus (HTLV) is the first unequivocal human retrovirus. Seroepidemiological and virus isolation studies indicate that HTLV is etiologically associated with a subtype of adult T-cell malignancy. We have molecularly cloned approximately 1 kilobase of sequences derived from the 5' and 3' termini of the HTLV genome. Use of these clones as probes allowed isolation of a 9.8-kilobase EcoRI fragment from a genomic DNA library of an HTLV-infected neoplastic T-cell line (CR). Analysis of this clone revealed the presence of cellular sequences flanking approximately 5 kilobases of viral sequences including one long terminal repeat sequence. The 5' and 3' clones, as well as subclones derived from different regions of the genomic clone, were used as probes to compare integrated proviruses and viral RNA expression in different HLTV-infected neoplastic T cell lines. The results indicate that the infected cells are of clonal origin with respect to the virus integration sites and they express multiple viral mRNA species including a 35S RNA.

MeSH Terms
Cell Line Cell Transformation, Viral Cloning, Molecular Defective Viruses/genetics Genetic Linkage Humans Repetitive Sequences, Nucleic Acid Retroviridae/genetics
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Manzari V
Wong-Staal F
Franchini G
Colombini S
Gelmann E P
Oroszlan S
Staal S
Gallo R C
References (35)
35 references, click to expand
  1. Mitogen requirements for the in vitro propagation of cutaneous T-cell lymphomas.
    Blood. 1980 Mar;55(3):409-17 PMID: 6244013
  2. An improved method for detecting foreign DNA in plasmids of Escherichia coli.
    Anal Biochem. 1979 Sep 15;98(1):60-3 PMID: 120689
  3. Activity of empty, headlike particles for packaging of DNA of bacteriophage lambda in vitro.
    Proc Natl Acad Sci U S A. 1974 Jun;71(6):2372-6 PMID: 4601587
  4. Selective in vitro growth of T lymphocytes from normal human bone marrows.
    Science. 1976 Sep 10;193(4257):1007-8 PMID: 181845
  5. Charon phages: safer derivatives of bacteriophage lambda for DNA cloning.
    Science. 1977 Apr 8;196(4286):161-9 PMID: 847462
  6. Screening lambdagt recombinant clones by hybridization to single plaques in situ.
    Science. 1977 Apr 8;196(4286):180-2 PMID: 322279
  7. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
    J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
  8. Levels of translatable mRNAs for cell surface protein, collagen precursors, and two membrane proteins are altered in Rous sarcoma virus-transformed chick embryo fibroblasts.
    Proc Natl Acad Sci U S A. 1977 Aug;74(8):3399-403 PMID: 198783
  9. Continuous growth and differentiation of human myeloid leukaemic cells in suspension culture.
    Nature. 1977 Nov 24;270(5635):347-9 PMID: 271272
  10. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
    Gene. 1977;2(2):95-113 PMID: 344137
  11. Retroviruses.
    Annu Rev Biochem. 1978;47:35-88 PMID: 354499
  12. Structure, replication, and recombination of retrovirus genomes: some unifying hypotheses.
    J Gen Virol. 1979 Jan;42(1):1-26 PMID: 215703
  13. Retrovirus sequences in a leukemic gibbon and its contact: evidence for partial provirus in the nonleukemic gibbon.
    Proc Natl Acad Sci U S A. 1979 Apr;76(4):2032-6 PMID: 287041
  14. Integration of bovine leukemia virus DNA in the bovine genome.
    Proc Natl Acad Sci U S A. 1979 Oct;76(10):4822-6 PMID: 228266
  15. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 PMID: 6159641
  16. T-cell lines established from human T-lymphocytic neoplasias by direct response to T-cell growth factor.
    Proc Natl Acad Sci U S A. 1980 Nov;77(11):6815-9 PMID: 6256763
  17. Analysis of avian leukosis virus DNA and RNA in bursal tumours: viral gene expression is not required for maintenance of the tumor state.
    Cell. 1981 Feb;23(2):311-22 PMID: 6258797
  18. Codon catalog usage is a genome strategy modulated for gene expressivity.
    Nucleic Acids Res. 1981 Jan 10;9(1):r43-74 PMID: 7208352
  19. Activation of a cellular onc gene by promoter insertion in ALV-induced lymphoid leukosis.
    Nature. 1981 Apr 9;290(5806):475-80 PMID: 6261142
  20. Detection and isolation of type C retrovirus particles from fresh and cultured lymphocytes of a patient with cutaneous T-cell lymphoma.
    Proc Natl Acad Sci U S A. 1980 Dec;77(12):7415-9 PMID: 6261256
  21. Characterization and distribution of nucleic acid sequences of a novel type C retrovirus isolated from neoplastic human T lymphocytes.
    Proc Natl Acad Sci U S A. 1981 Mar;78(3):1887-91 PMID: 6262827
  22. Immunological properties of a type C retrovirus isolated from cultured human T-lymphoma cells and comparison to other mammalian retroviruses.
    J Virol. 1981 Jun;38(3):906-15 PMID: 6264163
  23. Characterization of the reverse transcriptase from a new retrovirus (HTLV) produced by a human cutaneous T-cell lymphoma cell line.
    Virology. 1981 Jul 15;112(1):355-60 PMID: 6166122
  24. Molecular cloning of cDNA for the alpha, beta, and gamma chains of rat fibrinogen. A family of coordinately regulated genes.
    J Biol Chem. 1981 Sep 25;256(18):9718-23 PMID: 6169715
  25. Isolation of a new type C retrovirus (HTLV) in primary uncultured cells of a patient with Sézary T-cell leukaemia.
    Nature. 1981 Nov 19;294(5838):268-71 PMID: 6272125
  26. Antibodies in human sera reactive against an internal structural protein of human T-cell lymphoma virus.
    Nature. 1981 Nov 19;294(5838):271-3 PMID: 6272126
  27. Adult T-cell leukemia: antigen in an ATL cell line and detection of antibodies to the antigen in human sera.
    Proc Natl Acad Sci U S A. 1981 Oct;78(10):6476-80 PMID: 7031654
  28. Type C virus particles in a cord T-cell line derived by co-cultivating normal human cord leukocytes and human leukaemic T cells.
    Nature. 1981 Dec 24;294(5843):770-1 PMID: 6275274
  29. Adult T-cell lymphoma-leukaemia in Blacks from the West Indies.
    Lancet. 1982 Mar 20;1(8273):639-43 PMID: 6121963
  30. Primary structure analysis of the major internal protein p24 of human type C T-cell leukemia virus.
    Proc Natl Acad Sci U S A. 1982 Feb;79(4):1291-4 PMID: 6280175
  31. Natural antibodies to the structural core protein (p24) of the human T-cell leukemia (lymphoma) retrovirus found in sera of leukemia patients in Japan.
    Proc Natl Acad Sci U S A. 1982 Mar;79(5):1653-7 PMID: 6951204
  32. Isolation and characterization of retrovirus from cell lines of human adult T-cell leukemia and its implication in the disease.
    Proc Natl Acad Sci U S A. 1982 Mar;79(6):2031-5 PMID: 6979048
  33. A new subtype of human T-cell leukemia virus (HTLV-II) associated with a T-cell variant of hairy cell leukemia.
    Science. 1982 Nov 5;218(4572):571-3 PMID: 6981847
  34. Human T-cell leukemia-lymphoma virus (HTLV) is in T but not B lymphocytes from a patient with cutaneous T-cell lymphoma.
    Proc Natl Acad Sci U S A. 1982 Sep;79(18):5680-3 PMID: 6982476
  35. Natural antibodies to human retrovirus HTLV in a cluster of Japanese patients with adult T cell leukemia.
    Science. 1982 Feb 19;215(4535):975-8 PMID: 6760397
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.6.1574
Published
1983-03-00
Pages
1574-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC393644
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.80.6.1574 PMC Full Text View journal details
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