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PMID: 6092929 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Less than 40% of the simian virus 40 large T-antigen-coding sequence is required for transformation.

Molecular and cellular biology ·Vol. 4 ·No. 8 ·1984-08-00 ·Pages 1661-3

Sompayrac L, Danna KJ

Abstract

F8dl is a simian virus 40 early-region deletion mutant that lacks the simian virus 40 DNA sequences between 0.168 and 0.424 map units. Despite this large deletion, cloned F8dl DNA transforms Fisher rat F111 cells and BALB/3T3 clone A31 mouse cells as efficiently as does cloned simian virus 40 wild-type DNA. These results indicate that less than 40% of the large T-antigen-coding sequence is required for efficient transformation.

MeSH Terms
Animals Antigens, Neoplasm/analysis Antigens, Polyomavirus Transforming Antigens, Viral, Tumor/genetics Cell Line Cell Transformation, Neoplastic Cells, Cultured Chromosome Deletion Genes Genes, Viral Mice Mice, Inbred BALB C Mutation Rats Rats, Inbred F344 Simian virus 40/genetics Viral Proteins/genetics
Chemicals
Antigens, Neoplasm Antigens, Polyomavirus Transforming Antigens, Viral, Tumor Viral Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sompayrac L
Danna K J
References (10)
10 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.4.8.1661-1663.1984
Published
1984-08-00
Pages
1661-3
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC368965
Subset
IM
Grants
NCRR NIH HHS · 2 S07 RR07013-18 · United States
NCI NIH HHS · CA-34072 · United States
External Links
PubMed source DOI: 10.1128/mcb.4.8.1661-1663.1984 PMC Full Text View journal details
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