Home LiteratureArticle Details
PMID: 6300663 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Stabilization of the 53,000-dalton nonviral tumor antigen is not required for transformation by simian virus 40.

Molecular and cellular biology ·Vol. 3 ·No. 2 ·1983-02-00 ·Pages 290-6

Sompayrac LM, Gurney EG, Danna KJ

Abstract

We have isolated a simian virus 40 deletion mutant, F8dl, that lacks the sequences from 0.168 to 0.424 map units. The deleted sequences represent about one-half of the coding region for large T antigen. We present evidence here that F8dl is able to transform mouse cells in a focus assay and that cell lines derived from these foci exhibit fully transformed phenotypes, have integrated mutant genomes, and express mutant-encoded proteins. This result implies that the region of the simian virus 40 genome between 0.168 and 0.424 map units is not essential for the maintenance of transformation. In addition, we have found that cells fully transformed by F8dl produce a 53,000-dalton nonviral tumor antigen (p53) that is as unstable as the p53 of untransformed cells. From this result we infer that transformation by simian virus 40 does not require the stabilization of p53.

MeSH Terms
Animals Antigens, Neoplasm/genetics Cell Transformation, Viral Cells, Cultured Haplorhini Molecular Weight Simian virus 40/genetics
Chemicals
Antigens, Neoplasm
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Sompayrac L M
Gurney E G
Danna K J
References (32)
32 references, click to expand
  1. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  2. A new technique for the assay of infectivity of human adenovirus 5 DNA.
    Virology. 1973 Apr;52(2):456-67 PMID: 4705382
  3. Establishment and characterization of a cloned line of C3H mouse embryo cells sensitive to postconfluence inhibition of division.
    Cancer Res. 1973 Dec;33(12):3231-8 PMID: 4357355
  4. A nonselective analysis of SV40 transformation of mouse 3T3 cells.
    Virology. 1974 Jun;59(2):477-89 PMID: 4364825
  5. Altered patterns of protein synthesis in infection by SV40 mutants.
    Cold Spring Harb Symp Quant Biol. 1975;39 Pt 1:9-15 PMID: 169100
  6. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  7. Cell-free translation of simian virus 40 early messenger RNA coding for viral T-antigen.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):457-61 PMID: 191809
  8. A new method for sequencing DNA.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521
  9. Protein kinase activity associated with the avian sarcoma virus src gene product.
    Proc Natl Acad Sci U S A. 1978 Apr;75(4):2021-4 PMID: 205879
  10. Expression of simian virus 40 early genes in transformed rat cells is correlated with maintenance of the transformed phenotype.
    Proc Natl Acad Sci U S A. 1978 Oct;75(10):4987-91 PMID: 217009
  11. T antigen is bound to a host protein in SV40-transformed cells.
    Nature. 1979 Mar 15;278(5701):261-3 PMID: 218111
  12. Phosphorylation of T-antigen and control T-antigen expression in cells transformed by wild-type and tsA mutants of simian virus 40.
    J Virol. 1979 Feb;29(2):753-62 PMID: 219256
  13. DNA-mediated transfer of the adenine phosphoribosyltransferase locus into mammalian cells.
    Proc Natl Acad Sci U S A. 1979 Mar;76(3):1373-6 PMID: 286319
  14. Detection of a transformation-related antigen in chemically induced sarcomas and other transformed cells of the mouse.
    Proc Natl Acad Sci U S A. 1979 May;76(5):2420-4 PMID: 221923
  15. Identification of new polypeptide species (48-55K) immunoprecipitable by antiserum to purified large T antigen and present in SV40-infected and -transformed cells.
    Virology. 1979 Mar;93(2):466-80 PMID: 222051
  16. Characterization of a 54K dalton cellular SV40 tumor antigen present in SV40-transformed cells and uninfected embryonal carcinoma cells.
    Cell. 1979 May;17(1):43-52 PMID: 222475
  17. Identification and partial characterization of new antigens from simian virus 40-transformed mouse cells.
    J Virol. 1979 Aug;31(2):463-71 PMID: 225565
  18. Simian virus 40-transformed cells express new species of proteins precipitable by anti-simian virus 40 tumor serum.
    J Virol. 1979 Aug;31(2):472-83 PMID: 225566
  19. Characterization of different tumor antigens present in cells transformed by simian virus 40.
    Cell. 1979 Oct;18(2):335-46 PMID: 227604
  20. The SV40 A gene product is required for the production of a 54,000 MW cellular tumor antigen.
    Virology. 1979 Oct 30;98(2):308-18 PMID: 228475
  21. Host nuclear proteins expressed in simian virus 40-transformed and -infected cells.
    Proc Natl Acad Sci U S A. 1980 Jan;77(1):97-101 PMID: 6244576
  22. Association of a murine 53,000-dalton phosphoprotein with simian virus 40 large-T antigen in transformed cells.
    J Virol. 1980 Apr;34(1):213-24 PMID: 6246255
  23. Relationship among Tau antigens isolated from various lines of simian virus 40-transformed cells.
    J Virol. 1980 Jun;34(3):650-7 PMID: 6247503
  24. Monoclonal antibodies against simian virus 40 T antigens: evidence for distinct sublcasses of large T antigen and for similarities among nonviral T antigens.
    J Virol. 1980 Jun;34(3):752-63 PMID: 6155477
  25. Deletion mutants of SV40 that affect the structure of viral tumor antigens.
    Cold Spring Harb Symp Quant Biol. 1980;44 Pt 1,:285-91 PMID: 6253142
  26. Characterization of tau antigens isolated from uninfected and simian virus 40-infected monkey cells and papovavirus-transformed cells.
    J Virol. 1980 Nov;36(2):519-25 PMID: 6253675
  27. An embryo protein induced by SV40 virus transformation of mouse cells.
    Nature. 1980 Dec 25;288(5792):722-4 PMID: 6256642
  28. Efficient infection of monkey cells with DNA of simian virus 40.
    Proc Natl Acad Sci U S A. 1981 Dec;78(12):7575-8 PMID: 6278479
  29. Mutant of simian virus 40 large T-antigen that is defective for viral DNA synthesis, but competent for transformation of cultured rat cells.
    J Virol. 1982 Jun;42(3):854-64 PMID: 6285001
  30. Isolation and characterization of simian virus 40 early region deletion mutants.
    J Virol. 1982 Jul;43(1):328-31 PMID: 6287007
  31. A fragment of the SV40 large T-antigen gene transforms.
    Nature. 1982 Sep 2;299(5878):59-61 PMID: 6287280
  32. Post-translational regulation of the 54K cellular tumor antigen in normal and transformed cells.
    Mol Cell Biol. 1981 Feb;1(2):101-10 PMID: 6100960
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.3.2.290-296.1983
Published
1983-02-00
Pages
290-6
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC368533
Subset
IM
Grants
NCI NIH HHS · CA-21797 · United States
NCI NIH HHS · CA-24924 · United States
NCRR NIH HHS · RR07013-16 · United States
Databases
GENBANK
V00858
External Links
PubMed source DOI: 10.1128/mcb.3.2.290-296.1983 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2