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PMID: 6094472 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Identification of additional genes on transposon Tn10: tetC and tetD.

Journal of bacteriology ·Vol. 160 ·No. 2 ·1984-11-00 ·Pages 504-9

Braus G, Argast M, Beck CF

Abstract

Two genes (tetC and tetD) were identified and located on transposon Tn10 between gene tetA and insertion sequence IS10R. Genes tetC and tetD encode proteins of apparent subunit molecular weights of 23,000 and 18,000, respectively. The TetD protein was found to be membrane associated. Tetracycline resistance levels promoted by transposon Tn10 were found to be unaffected in Escherichia coli K-12 when mutants lacking tetC or tetC and tetD were tested. The nucleotide sequence of genes tetC and tetD is reported in the accompanying article (K. Schollmeier and W. Hillen, J. Bacteriol. 160:499-503, 1984).

MeSH Terms
Bacterial Proteins/isolation & purification Base Sequence DNA Restriction Enzymes DNA Transposable Elements Drug Resistance, Microbial Escherichia coli/drug effects,genetics Genes Genes, Bacterial Macromolecular Substances Molecular Weight Mutation Plasmids Tetracycline/pharmacology
Chemicals
Bacterial Proteins DNA Transposable Elements Macromolecular Substances DNA Restriction Enzymes Tetracycline
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Braus G
Argast M
Beck C F
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32 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.160.2.504-509.1984
Published
1984-11-00
Pages
504-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC214762
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.160.2.504-509.1984 PMC Full Text View journal details
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