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PMID: 286147 Published · ppublish English Journal Article

In vitro constructed plasmids containing both ends of bacteriophage Mu DNA express phage functions.

Molecular & general genetics : MGG ·Vol. 169 ·No. 1 ·1979-01-16 ·Pages 97-105

Schumann W, Bade EG

Abstract

The construction of a plasmid carrying the right end PstI . B fragment of bacteriophage Mu DNA and of plasmids containing in addition the left end EcoRI.C fragment of Mu DNA into the vector pBR322 is described. Inversion of the G segment still occurs in all these plasmids. By marker rescue and complementation experiments the right PstI cleavage site was located to the left of gene Q. The composite plasmids inheriting also the left end EcoRI fragment of Mu DNA express both the immunity and killing functions of Mu and direct the in vitro synthesis of presumably Mu-specific polypeptides. These results demonstrates that Mu-specific functions can be analyzed from cloned fragments.

MeSH Terms
Coliphages/genetics,metabolism DNA Restriction Enzymes/genetics DNA, Recombinant DNA, Viral/genetics Genes, Viral Plasmids Viral Proteins/biosynthesis
Chemicals
DNA, Recombinant DNA, Viral Viral Proteins DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Schumann W
Bade E G
References (34)
34 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00267550
Published
1979-01-16
Pages
97-105
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00267550
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