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PMID: 6094474 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

DNA sequence and transcriptional organization of essential cell division genes ftsQ and ftsA of Escherichia coli: evidence for overlapping transcriptional units.

Journal of bacteriology ·Vol. 160 ·No. 2 ·1984-11-00 ·Pages 546-55

Robinson AC, Kenan DJ, Hatfull GF, Sullivan NF, Spiegelberg R, Donachie WD

Abstract

The DNA sequence of a cloned segment of the Escherichia coli chromosome containing ftsQ, ftsA, and part of the ftsZ gene was determined and interpreted for genetic complementation and promoter fusion data for the region. The contiguous genes ftsQ, ftsA, and ftsZ were transcribed in the same direction (clockwise on the genetic map) and each had at least one associated promoter which allowed it to be transcribed independently of neighboring genes. ftsA and ftsZ possessed promoters within the coding sequences of the juxtaposed upstream structural genes, and a promoter element for ftsA was surrounded by a region of twofold symmetry which corresponded closely to a symmetrical element in the region of a putative ftsZ promoter. The structural gene of ftsQ consisted of 838 nucleotides, encoding a 276-residue amino acid polypeptide of molecular weight 31,400; the structural gene of ftsA consisted of 1,260 nucleotides, encoding a 420-residue amino acid polypeptide of molecular weight 45,400. The observation that the termination codon of ftsQ overlaps with a potential initiation codon for ftsA suggested that these two genes may be translationally coupled when transcription is initiated upstream of the ftsQ coding sequence.

MeSH Terms
Amino Acid Sequence Base Sequence Cell Division Cloning, Molecular DNA Restriction Enzymes DNA, Bacterial/genetics Escherichia coli/cytology,genetics Genes Genes, Regulator Plasmids Promoter Regions, Genetic Transcription, Genetic
Chemicals
DNA, Bacterial DNA Restriction Enzymes
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Robinson A C
Kenan D J
Hatfull G F
Sullivan N F
Spiegelberg R
Donachie W D
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44 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.160.2.546-555.1984
Published
1984-11-00
Pages
546-55
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC214769
Subset
IM
Databases
GENBANK
K02668
External Links
PubMed source DOI: 10.1128/jb.160.2.546-555.1984 PMC Full Text View journal details
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