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PMID: 6094486 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Restoration of viability to an Escherichia coli mutant deficient in the 5'----3' exonuclease of DNA polymerase I.

Journal of bacteriology ·Vol. 160 ·No. 2 ·1984-11-00 ·Pages 706-10

Boling M, Adler H, Masker W

Abstract

An Escherichia coli polA (Ex) mutant that is usually inviable at restrictive temperatures (43 degrees C) was found to grow normally at 43 degrees C when incubated in the presence of a membrane-containing fraction prepared from E. coli. This membrane fraction causes anaerobic conditions that are necessary but not sufficient for restoration of viability since some component present in the membrane fraction is also required for colony formation at 43 degrees C. The accumulation of small DNA fragments typical of aerobic growth of the polA(Ex) mutant was also seen under anaerobic conditions. The polA(Ex) strain was also much more sensitive than the isogenic wild-type strain to hydrogen peroxide.

MeSH Terms
Anaerobiosis Chromosome Deletion DNA Polymerase I/genetics DNA Replication Drug Resistance Escherichia coli/drug effects,enzymology,genetics Exonucleases/genetics Genes Genes, Bacterial Mutation Potassium Chloride/pharmacology Sodium Chloride/pharmacology Temperature
Chemicals
Sodium Chloride Potassium Chloride DNA Polymerase I Exonucleases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Boling M
Adler H
Masker W
References (18)
18 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.160.2.706-710.1984
Published
1984-11-00
Pages
706-10
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC214794
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.160.2.706-710.1984 PMC Full Text View journal details
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