Home LiteratureArticle Details
PMID: 6095062 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Positive regulatory interactions of the HIS4 gene of Saccharomyces cerevisiae.

Molecular and cellular biology ·Vol. 4 ·No. 7 ·1984-07-00 ·Pages 1326-33

Lucchini G, Hinnebusch AG, Chen C, Fink GR

Abstract

The role of cis- and trans-acting elements in the expression of HIS4 has been examined by using HIS4-lacZ fusions in which lacZ expression is dependent upon the HIS4 5' noncoding region. The cis-acting sequences involved in regulation were defined by studying the effects of the wild-type and various deletions and their revertants on regulation via the general control of amino acid biosynthesis. The role of trans-acting genes was analyzed by studying the regulation of the HIS4-lacZ fusions in strains carrying mutations in the GCN (AAS) or GCD (TRA) genes and in strains carrying the GCN genes on high-copy-number plasmids. These studies have led to the following conclusions. (i) HIS4 is positively regulated by the general control. (ii) At least one copy of the 5'TGACTC3' repeat at -136 is required in cis for this regulation. (iii) Both the GCN4 gene and at least one copy of the repeated sequence are required for expression at the repressed level. (iv) The open reading frames in the 5' noncoding region are not required in either cis or trans for the regulation of HIS4.

MeSH Terms
Chromosome Deletion DNA Restriction Enzymes Genes Genes, Fungal Genes, Regulator Mutation Plasmids Saccharomyces cerevisiae/genetics Species Specificity beta-Galactosidase/genetics
Chemicals
DNA Restriction Enzymes beta-Galactosidase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Lucchini G
Hinnebusch A G
Chen C
Fink G R
References (22)
22 references, click to expand
  1. Regulation of tryptophan biosynthesis in Saccharomyces cerevisiae: mode of action of 5-methyl-tryptophan and 5-methyl-tryptophan-sensitive mutants.
    J Bacteriol. 1974 Mar;117(3):1131-40 PMID: 4360539
  2. The regulation of arginine biosynthesis in Saccharomyces cerevisiae. The specificity of argR- mutations and the general control of amino-acid biosynthesis.
    Eur J Biochem. 1975 Sep 1;57(1):231-9 PMID: 1100402
  3. Integration of amino acid biosynthesis into the cell cycle of Saccharomyces cerevisiae.
    J Mol Biol. 1975 Aug 5;96(2):273-90 PMID: 1100845
  4. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
    Anal Biochem. 1976 May 7;72:248-54 PMID: 942051
  5. Transformation of yeast.
    Proc Natl Acad Sci U S A. 1978 Apr;75(4):1929-33 PMID: 347451
  6. Tryptophan biosynthesis in Saccharomyces cerevisiae: control of the flux through the pathway.
    J Bacteriol. 1978 Apr;134(1):48-59 PMID: 348687
  7. Transformation in yeast: development of a hybrid cloning vector and isolation of the CAN1 gene.
    Gene. 1979 Dec;8(1):121-33 PMID: 395029
  8. Sterile host yeasts (SHY): a eukaryotic system of biological containment for recombinant DNA experiments.
    Gene. 1979 Dec;8(1):17-24 PMID: 395030
  9. Yeast genes fused to beta-galactosidase in Escherichia coli can be expressed normally in yeast.
    Proc Natl Acad Sci U S A. 1981 Apr;78(4):2460-4 PMID: 6787605
  10. Yeast gene TRP5: structure, function, regulation.
    J Biol Chem. 1982 Feb 10;257(3):1491-500 PMID: 6276387
  11. Transcription of the his3 gene region in Saccharomyces cerevisiae.
    J Mol Biol. 1981 Nov 5;152(3):535-52 PMID: 6173489
  12. The nucleotide sequence of the HIS4 region of yeast.
    Gene. 1982 Apr;18(1):47-59 PMID: 7049842
  13. Regulatory sites for his3 gene expression in yeast.
    Nature. 1982 Nov 18;300(5889):285-6 PMID: 6755264
  14. Regulation of HIS4-lacZ fusions in Saccharomyces cerevisiae.
    Mol Cell Biol. 1982 Oct;2(10):1212-9 PMID: 6817079
  15. Isolation of the yeast regulatory gene GAL4 and analysis of its dosage effects on the galactose/melibiose regulon.
    Proc Natl Acad Sci U S A. 1982 Nov;79(22):6971-5 PMID: 6294669
  16. A short nucleotide sequence required for regulation of HIS4 by the general control system of yeast.
    Cell. 1983 Jan;32(1):89-98 PMID: 6337724
  17. Repeated DNA sequences upstream from HIS1 also occur at several other co-regulated genes in Saccharomyces cerevisiae.
    J Biol Chem. 1983 Apr 25;258(8):5238-47 PMID: 6300123
  18. Identification of AAS genes and their regulatory role in general control of amino acid biosynthesis in yeast.
    Proc Natl Acad Sci U S A. 1983 May;80(9):2704-8 PMID: 6341997
  19. Participation of transcriptional and post-transcriptional regulatory mechanisms in the control of arginine metabolism in yeast.
    Mol Gen Genet. 1983;189(1):148-56 PMID: 6343780
  20. Positive regulation in the general amino acid control of Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1983 Sep;80(17):5374-8 PMID: 6351059
  21. Structure and function of the yeast URA3 gene. Differentially regulated expression of hybrid beta-galactosidase from overlapping coding sequences in yeast.
    J Mol Biol. 1983 Nov 15;170(4):883-904 PMID: 6315953
  22. Biological role of the general control of amino acid biosynthesis in Saccharomyces cerevisiae.
    Mol Cell Biol. 1981 Jul;1(7):584-93 PMID: 9279372
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.4.7.1326-1333.1984
Published
1984-07-00
Pages
1326-33
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC368915
Subset
IM
Grants
NCI NIH HHS · CA34429 · United States
NIGMS NIH HHS · GM31802 · United States
External Links
PubMed source DOI: 10.1128/mcb.4.7.1326-1333.1984 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2