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PMID: 6096692 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Role of intron-contained sequences in formation of moloney murine leukemia virus env mRNA.

Molecular and cellular biology ·Vol. 4 ·No. 11 ·1984-11-00 ·Pages 2289-97

Hwang LS, Park J, Gilboa E

Abstract

Formation of the Moloney murine leukemia virus envelope mRNA involves the removal of a 5,185-base pair-long intron. Deletion analysis of two Moloney murine leukemia virus-derived expression vectors revealed the existence of two short regions within the viral intron which are required for the efficient formation of the spliced RNA species. One region was present upstream from the 3' splice junction, extended at least 85 nucleotides beyond the splice site, and was not more than 165 nucleotides long. As yeast polymerase II introns, the Moloney murine leukemia virus intron contains the sequence 5'-TACTAAC-3' 15 nucleotides upstream from the 3' splice site. A second region located in the middle of the intron, within a 560-nucleotide-long sequence, was also essential for formation of the spliced RNA species. The efficient splicing of the env mRNA in the absence of expression of viral genes raises the possibility that similar mechanisms are used to remove introns of (some) cellular genes.

MeSH Terms
Base Sequence Genes, Viral Genetic Vectors Moloney murine leukemia virus/genetics RNA Splicing RNA, Messenger/genetics RNA, Viral/genetics
Chemicals
RNA, Messenger RNA, Viral
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hwang L S
Park J
Gilboa E
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.4.11.2289-2297.1984
Published
1984-11-00
Pages
2289-97
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC369057
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.4.11.2289-2297.1984 PMC Full Text View journal details
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