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PMID: 6098471 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Protein fusions with the kanamycin resistance gene from transposon Tn5.

The EMBO journal ·Vol. 3 ·No. 13 ·1984-12-20 ·Pages 3317-22

Reiss B, Sprengel R, Schaller H

Abstract

The gene for the neomycin phosphotransferase II (NPT II) from transposon Tn5 was fused at the amino or carboxy terminus to foreign DNA sequences coding for 3-300 amino acids and the properties of the fused proteins were investigated. All amino-terminal fusions examined conferred kanamycin resistance to their host cell, but profound differences in their enzymatic activity and stability were detected. Short additions to the amino terminus of the NPT II resulted in highly enzymatically active fusion proteins whereas long amino-terminal fusions often had to be proteolytically degraded to release active proteins. Fusions at the carboxy-terminal end of the NPT II protein did not always induce kanamycin resistance and their enzymatic activity depended more stringently on the nature of the junction sequence.

MeSH Terms
Base Sequence DNA Transposable Elements DNA, Recombinant Drug Resistance, Microbial Escherichia coli/genetics Genes, Bacterial Kanamycin/pharmacology Kanamycin Kinase Phosphotransferases/genetics Plasmids
Chemicals
DNA Transposable Elements DNA, Recombinant Kanamycin Phosphotransferases Kanamycin Kinase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Reiss B
Sprengel R
Schaller H
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1984-12-20
Pages
3317-22
Language
English
Region
England
NLM ID
8208664
PMCID
PMC557856
Subset
IM
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