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PMID: 6107906 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Isolation and structure of pro-somatostatin: a putative somatostatin precursor from pig hypothalamus.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 8 ·1980-08-00 ·Pages 4489-93

Schally AV, Huang WY, Chang RC, Arimura A, Redding TW, Millar RP, Hunkapiller MW, Hood LE

Abstract

An octacosapeptide that we named pro-somatostatin has been isolated from acid extracts of porcine hypothalami and found to have the amino acid sequence Ser-Ala-Asn-Ser-Asn-Pro-Ala-Met-Ala-Pro-Arg-Glu-Arg-Lys-Ala-Gly-Cys-Lys-Asn-Phe-Phe-Trp-Lys-Thr-Phe-Thr-Ser-Cys. This octacosapeptide possesses high somatotropin (growth hormone) and prolactin release-inhibiting activity in vitro. It also crossreacts strongly with antisera generated against the somatostatin tetradecapeptide. This octacosapeptide is most likely a precursor (pro-hormone) of somatostatin in the hypothalamus. The existence of still larger molecular size precursors of somatostatin was also observed.

MeSH Terms
Amino Acid Sequence Amino Acids/analysis Animals Biological Assay Chromatography/methods Hypothalamus/metabolism Protein Precursors/isolation & purification Somatostatin/immunology,metabolism Structure-Activity Relationship Swine
Chemicals
Amino Acids Protein Precursors Somatostatin
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Schally A V
Huang W Y
Chang R C
Arimura A
Redding T W
Millar R P
Hunkapiller M W
Hood L E
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.77.8.4489
Published
1980-08-00
Pages
4489-93
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC349869
Subset
IM
Grants
NIADDK NIH HHS · AM-07467 · United States
NIADDK NIH HHS · AM-09094 · United States
NIGMS NIH HHS · GM-06965 · United States
External Links
PubMed source DOI: 10.1073/pnas.77.8.4489 PMC Full Text View journal details
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