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PMID: 6117826 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Nucleotide sequence of the asnA gene coding for asparagine synthetase of E. coli K-12.

Nucleic acids research ·Vol. 9 ·No. 18 ·1981-09-25 ·Pages 4669-76

Nakamura M, Yamada M, Hirota Y, Sugimoto K, Oka A, Takanami M

Abstract

We have subcloned the asnA gene of E. coli K-12, a gene coding for asparagine synthetase, from a previously cloned 6 mega-dalton segment of E. coli chromosome containing the DNA replication origin, ori, and asnA. The complete nucleotide sequence of the asnA gene was determined: the region of the structural gene extends 990 base-pairs at nucleotide positions 1434-2423 (see Fig. 3), which codes for a polypeptide of 330 amino-acid residues with a molecular weight of 36,688 daltons. The nucleotide sequences of the promoter and the ribosome-binding site of the gene are also assigned. We discuss the properties of its polypeptide.

MeSH Terms

Amino Acid Sequence Aspartate-Ammonia Ligase/genetics Base Composition Base Sequence Cloning, Molecular Codon DNA Replication DNA Restriction Enzymes DNA, Bacterial/genetics Escherichia coli/enzymology,genetics Genes Ligases/genetics

Chemicals

Codon DNA, Bacterial DNA Restriction Enzymes Ligases Aspartate-Ammonia Ligase

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Nakamura M

Yamada M

Hirota Y

Sugimoto K

Oka A

Takanami M

References (24)

24 references, click to expand

Studies on polynucleotides. LXXV. Specificity of tRNA for codon recognition as studied by the ribosomal binding technique.
J Mol Biol. 1967 Oct 14;29(1):97-112 PMID: 4861614
Nucleotide sequences of trpA of Salmonella typhimurium and Escherichia coli: an evolutionary comparison.
Proc Natl Acad Sci U S A. 1979 Oct;76(10):5244-8 PMID: 388433
Genetic and biomedical studies demonstrating a second gene coding for asparagine synthetase in Escherichia coli.
J Bacteriol. 1980 Apr;142(1):212-20 PMID: 6102982
Mutations in two unlinked genes are required to produce asparagine auxotrophy in Escherichia coli.
J Bacteriol. 1980 Apr;142(1):221-8 PMID: 6102983
Linkage map of Escherichia coli K-12, edition 6.
Microbiol Rev. 1980 Mar;44(1):1-56 PMID: 6997720
Codon catalog usage is a genome strategy modulated for gene expressivity.
Nucleic Acids Res. 1981 Jan 10;9(1):r43-74 PMID: 7208352
The DNA replication origin (ori) of Escherichia coli: structure and function of the ori-containing DNA fragment.
Prog Nucleic Acid Res Mol Biol. 1981;26:33-48 PMID: 6169112
The asparagine synthetase of Escherhic coli. I. Biosynthetic role of the enzyme, purification, and characterization of the reaction products.
J Biol Chem. 1969 Aug 10;244(15):4112-21 PMID: 4895361
The sequence of nucleotides in tRNA Ile from E. coli B.
Biochem Biophys Res Commun. 1971 May 21;43(4):729-34 PMID: 4935283
Purification of five leucine transfer ribonucleic acid species from Escherichia coli and their acylation by heterologous leucyl-transfer ribonucleic acid synthetase.
J Biol Chem. 1971 Aug 25;246(16):4947-50 PMID: 4936719
Studies on the mechanism of repression of arginine biosynthesis in Escherichia coli. IV. Further studies on the role of arginine transfer RNA repression of the enzymes of arginine biosynthesis.
J Mol Biol. 1971 Nov 28;62(1):179-88 PMID: 4945528
Specific cleavage of coliphage fd DNA by five different restriction endonucleases from Haemophilus genus.
FEBS Lett. 1973 Aug 15;34(2):318-22 PMID: 4542920
Transformation of Salmonella typhimurium by plasmid deoxyribonucleic acid.
J Bacteriol. 1974 Sep;119(3):1072-4 PMID: 4605400
Membrane proteins: amino acid sequence and membrane penetration.
J Mol Biol. 1974 Aug 25;87(4):853-8 PMID: 4427380
A new method for sequencing DNA.
Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521
Spacer transfer RNAs in ribosomal RNA transcripts of E. coli: processing of 30S ribosomal RNA in vitro.
Cell. 1977 Jun;11(2):247-62 PMID: 329997
Cloning and mapping of the replication origin of Escherichia coli.
Proc Natl Acad Sci U S A. 1977 Dec;74(12):5458-62 PMID: 341158
Primary structure of tRNA-Lys of E. coli B.
Nucleic Acids Res. 1975 Nov;2(11):2069-75 PMID: 802509
Bacteriophage lambda carrying the Escherichia coli chromosomal region of the replication origin.
Proc Natl Acad Sci U S A. 1978 Oct;75(10):5099-103 PMID: 368808
Nucleotide sequence of Escherichia coli K-12 replication origin.
Proc Natl Acad Sci U S A. 1979 Feb;76(2):575-9 PMID: 370832
Nucleotide sequence of the origin of replication of the Escherichia coli K-12 chromosome.
Proc Natl Acad Sci U S A. 1979 Feb;76(2):580-4 PMID: 370833
Nucleotide sequence of the ribosomal protein gene cluster adjacent to the gene for RNA polymerase subunit beta in Escherichia coli.
Proc Natl Acad Sci U S A. 1979 Apr;76(4):1697-701 PMID: 377281
Structural and functional properties of the Escherichia coli origin of DNA replication.
Cold Spring Harb Symp Quant Biol. 1979;43 Pt 1:129-38 PMID: 383377
Regulatory sequences involved in the promotion and termination of RNA transcription.
Annu Rev Genet. 1979;13:319-53 PMID: 94251

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/9.18.4669

Published

1981-09-25

Pages

4669-76

Language

English

Region

England

NLM ID

0411011

PMCID

PMC327466

Subset

Databases

GENBANK

J01657, X02820

External Links

PubMed source DOI: 10.1093/nar/9.18.4669 PMC Full Text View journal details

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Nucleotide sequence of the asnA gene coding for asparagine synthetase of E. coli K-12.

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