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PMID: 6124506 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Antibodies against the Ag2 fimbriae of Actinomyces viscosus T14V inhibit lactose-sensitive bacterial adherence.

Infection and immunity ·Vol. 36 ·No. 3 ·1982-06-00 ·Pages 1217-22

Revis GJ, Vatter AE, Crowle AJ, Cisar JO

Abstract

Monospecific antisera against the Ag1 and Ag2 fimbrial components of Actinomyces viscosus T14V were produced by immunizing rabbits with immune precipitates of each antigen harvested from crossed-immunoelectrophoresis plates. The Fab fragments prepared from these sera were used as specific reagents in immunoelectron microscopy to identify each fimbrial component on the bacterial surface and also were assayed for their abilities to prevent the coaggregation of A. viscosus T14V with Streptococcus sanguis 34, an interaction that is lactose sensitive. Each Fab preparation appeared to react with different fibrillar structures present on the actinomycete, and only the Ag2-specific Fabs blocked coaggregation. These results provide strong support for the presence of distinct types of fimbriae on A. viscosus T14V and indicate the exclusive involvement of Ag2 fimbriae in lactose-sensitive adherence.

MeSH Terms
Actinomyces/immunology Antibodies, Bacterial/immunology Antibody Specificity Antigens, Bacterial/immunology Binding, Competitive Cell Adhesion Fimbriae, Bacterial/immunology Immunoglobulin Fab Fragments Lactose/metabolism Streptococcus sanguis/immunology
Chemicals
Antibodies, Bacterial Antigens, Bacterial Immunoglobulin Fab Fragments Lactose
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Revis G J
Vatter A E
Crowle A J
Cisar J O
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26 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.36.3.1217-1222.1982
Published
1982-06-00
Pages
1217-22
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC551459
Subset
IM
Grants
NIDCR NIH HHS · R01 DE05156 · United States
External Links
PubMed source DOI: 10.1128/iai.36.3.1217-1222.1982 PMC Full Text View journal details
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