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PMID: 7399676 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Fibril-mediated adherence of Actinomyces viscosus to saliva-treated hydroxyapatite.

Infection and immunity ·Vol. 28 ·No. 2 ·1980-05-00 ·Pages 577-84

Wheeler TT, Clark WB

Abstract

Fibril-mediated adherence of Actinomyces viscosus strain T14V cells to saliva-treated hydroxyapatite was studied. Fibrils were purified by ammonium sulfate precipitation and differential centrifugation from the crude supernatant of whole cells that were sheared by one passage through a French pressure cell. Purified fibrils and crude supernatant inhibited strain T14V adherence to saliva-treated hydroxyapatite to similar extents. However, anti-strain T14V serum and antifibril specific antibody completely abolished strain T14V adherence. The blocking immunoglobulin could be adsorbed from anti-T14V serum by strain T14V whole cells, by purified fibrils, and, to a lesser extent, by cell walls. It was concluded that fibrils mediate adherence of strain T14V cells to saliva-treated hydroxyapatite. In addition, fibril preparations were shown to contain more than 95% protein and to be antigenically homogeneous by immunodiffusion and Laurell rocket immunoelectrophoresis. Purified fibril preparations showed serological identity with the virulence-associated 1 antigen of Lancefield-extracted T14V cells, whereas crude supernatants contained both virulence-associated 1 and virulence-associated 2 antigens, as shown by rocket immunoelectrophoresis.

MeSH Terms
Actinomyces/ultrastructure Amino Acids/analysis Amyloid/immunology Animals Antigens Electrophoresis, Polyacrylamide Gel Humans Hydroxyapatites/immunology Immune Sera Microscopy, Electron Rabbits Saliva/microbiology Serum Amyloid A Protein/immunology
Chemicals
Amino Acids Amyloid Antigens Hydroxyapatites Immune Sera Serum Amyloid A Protein
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wheeler T T
Clark W B
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48 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.28.2.577-584.1980
Published
1980-05-00
Pages
577-84
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC550973
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.28.2.577-584.1980 PMC Full Text View journal details
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