Home LiteratureArticle Details

PMID: 6140260 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Nucleotide sequence of the papA gene encoding the Pap pilus subunit of human uropathogenic Escherichia coli.

Journal of bacteriology ·Vol. 157 ·No. 1 ·1984-01-00 ·Pages 330-3

Båga M, Normark S, Hardy J, O'Hanley P, Lark D, Olsson O, Schoolnik G, Falkow S

Abstract

The papA gene of the uropathogenic strain Escherichia coli J96, coding for the Pap pili subunit, was subjected to DNA sequencing, and found to code for an 185-amino acid-long polypeptide with a 22-amino acid-long signal peptide. Here we present the primary sequence, the hydrophilicity profile, and the predicted polypeptide secondary structure of the Pap pili subunit.

MeSH Terms

Amino Acid Sequence Base Sequence DNA, Bacterial/genetics Escherichia coli/genetics,ultrastructure Fimbriae, Bacterial/ultrastructure Genes, Bacterial Genetic Code Humans Peptides/genetics Protein Conformation Urologic Diseases/microbiology

Chemicals

DNA, Bacterial Peptides

Authors & Affiliations

8 authors, click to expand affiliations / ORCID

Båga M

Normark S

Hardy J

O'Hanley P

Lark D

Olsson O

Schoolnik G

Falkow S

References (24)

24 references, click to expand

Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels.
J Mol Biol. 1977 Feb 15;110(1):119-46 PMID: 845942
Genetics of digalactoside-binding adhesin from a uropathogenic Escherichia coli strain.
Infect Immun. 1983 Sep;41(3):942-9 PMID: 6136465
DNA sequencing with chain-terminating inhibitors.
Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteins.
J Mol Biol. 1978 Mar 25;120(1):97-120 PMID: 642007
Adhesion to normal human uroepithelial cells of Escherichia coli from children with various forms of urinary tract infection.
J Pediatr. 1978 Sep;93(3):398-403 PMID: 357693
Hemagglutination patterns of enterotoxigenic and enteropathogenic Escherichia coli determined with human, bovine, chicken, and guinea pig erythrocytes in the presence and absence of mannose.
Infect Immun. 1979 Feb;23(2):336-46 PMID: 370014
E. coli RNA polymerase interacts homologously with two different promoters.
Cell. 1980 Jun;20(2):269-81 PMID: 6248238
Secretion and membrane localization of proteins in Escherichia coli.
CRC Crit Rev Biochem. 1980;7(4):339-71 PMID: 6993100
Identification of a carbohydrate receptor recognized by uropathogenic Escherichia coli.
Infection. 1980;8 Suppl 3:288-93 PMID: 6997213
Adhesion, hemagglutination, and virulence of Escherichia coli causing urinary tract infections.
Infect Immun. 1981 Feb;31(2):564-70 PMID: 7012012
Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing.
J Mol Biol. 1980 Oct 25;143(2):161-78 PMID: 6260957
Bacterial adherence: adhesin-receptor interactions mediating the attachment of bacteria to mucosal surface.
J Infect Dis. 1981 Mar;143(3):325-45 PMID: 7014727
Prediction of protein antigenic determinants from amino acid sequences.
Proc Natl Acad Sci U S A. 1981 Jun;78(6):3824-8 PMID: 6167991
Structure of carbohydrate part of receptor on human uroepithelial cells for pyelonephritogenic Escherichia coli.
Lancet. 1981 Sep 19;2(8247):604-6 PMID: 6116085
The complete amino-acid sequence of the K88 antigen, a fimbrial protein from Escherichia coli.
Eur J Biochem. 1981 Jul;117(3):617-27 PMID: 7026236
Construction and expression of recombinant plasmids encoding type 1 or D-mannose-resistant pili from a urinary tract infection Escherichia coli isolate.
Infect Immun. 1981 Sep;33(3):933-8 PMID: 6116675
Translational initiation in prokaryotes.
Annu Rev Microbiol. 1981;35:365-403 PMID: 6170248
Common evolutionary origin of chromosomal beta-lactamase genes in enterobacteria.
J Bacteriol. 1982 May;150(2):528-34 PMID: 6802795
Correlation between the abundance of Escherichia coli transfer RNAs and the occurrence of the respective codons in its protein genes: a proposal for a synonymous codon choice that is optimal for the E. coli translational system.
J Mol Biol. 1981 Sep 25;151(3):389-409 PMID: 6175758
F7 and type 1-like fimbriae from three Escherichia coli strains isolated from urinary tract infections: protein chemical and immunological aspects.
Infect Immun. 1982 May;36(2):462-8 PMID: 6123483
Primary structure of the CFA1 fimbrial protein from human enterotoxigenic Escherichia coli strains.
Eur J Biochem. 1982 May 17;124(2):339-48 PMID: 6124420
A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments.
Gene. 1982 Oct;19(3):269-76 PMID: 6295880
A pilus peptide vaccine for the prevention of gonorrhea.
Prog Allergy. 1983;33:314-31 PMID: 6187017
Variable adherence to normal human urinary-tract epithelial cells of Escherichia coli strains associated with various forms of urinary-tract infection.
Lancet. 1976 Sep 4;1(7984):490-2 PMID: 74461

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.157.1.330-333.1984

Published

1984-01-00

Pages

330-3

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC215181

Subset

Grants

PHS HHS · A310885-08 · United States

Databases

GENBANK

K01176, X03391, X03392

External Links

PubMed source DOI: 10.1128/jb.157.1.330-333.1984 PMC Full Text View journal details

Analysis Services

Nucleotide sequence of the papA gene encoding the Pap pilus subunit of human uropathogenic Escherichia coli.

Contact

WeChat Official Account