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PMID: 6141143 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Specific inhibition of adsorption of Actinomyces viscosus T14V to saliva-treated hydroxyapatite by antibody against type 1 fimbriae.

Infection and immunity ·Vol. 43 ·No. 2 ·1984-02-00 ·Pages 497-501

Clark WB, Wheeler TT, Cisar JO

Abstract

Specific antibodies and their Fab fragments were used to study adsorption of Antinomyces viscosus T14V to saliva-treated hydroxyapatite. Antibody against A. viscosus T14V blocked adsorption of the strain to saliva-treated hydroxyapatite, and this activity was removed after preincubation of the immune immunoglobulin G (IgG) with purified type 1 fimbriae in amounts near equivalence but not after preincubation with type 2 fimbriae in antigen excess. Specific IgG or Fab fragments against type 1 fimbriae inhibited adsorption of strain T14V to saliva-treated hydroxyapatite and promoted desorption of cells already attached to the apatite surface. Anti-type 2 IgG or Fab fragments did not possess these activities. These data demonstrate that type 1 but not type 2 fimbriae mediate adsorption of strain T14V to the saliva-treated hydroxyapatite surface.

MeSH Terms
Actinomyces/immunology Antibodies, Bacterial/immunology Durapatite Enzyme-Linked Immunosorbent Assay Fimbriae, Bacterial/immunology Humans Hydroxyapatites Immunoglobulin Fab Fragments/immunology Immunoglobulin G/immunology Immunosorbent Techniques Saliva
Chemicals
Antibodies, Bacterial Hydroxyapatites Immunoglobulin Fab Fragments Immunoglobulin G Durapatite
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Clark W B
Wheeler T T
Cisar J O
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20 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.43.2.497-501.1984
Published
1984-02-00
Pages
497-501
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC264324
Subset
IM
Grants
NIDCR NIH HHS · DE-05429 · United States
NIDCR NIH HHS · DE00115 · United States
External Links
PubMed source DOI: 10.1128/iai.43.2.497-501.1984 PMC Full Text View journal details
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