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PMID: 6144542 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Properties of acetylcholine receptors translated by cat muscle mRNA in Xenopus oocytes.

The EMBO journal ·Vol. 1 ·No. 11 ·1982-00-00 ·Pages 1307-12

Miledi R, Parker I, Sumikawa K

Abstract

Poly(A)+ mRNA, extracted from denervated skeletal muscles of the cat, directs the synthesis of acetylcholine receptors in Xenopus laevis oocytes. The receptors are inserted in the oocyte membrane where they form acetylcholine receptor-channel complexes which have properties like those of the native receptors in the muscle membrane.

MeSH Terms

Animals Cats Female Muscle Denervation Muscles/metabolism Oocytes/physiology Poly A/genetics Protein Biosynthesis RNA, Messenger/genetics Receptors, Cholinergic/genetics Receptors, Muscarinic/physiology Receptors, Nicotinic/physiology Xenopus

Chemicals

RNA, Messenger Receptors, Cholinergic Receptors, Muscarinic Receptors, Nicotinic Poly A

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Miledi R

Parker I

Sumikawa K

References (17)

17 references, click to expand

Voltage clamp analysis of acetylcholine produced end-plate current fluctuations at frog neuromuscular junction.
J Physiol. 1973 Dec;235(3):655-91 PMID: 4543940
The effect of procaine on the action of acetylcholine at the neuromuscular junction.
J Physiol. 1975 Jul;249(2):269-84 PMID: 1177094
Structural gene sets active in embryos and adult tissues of the sea urchin.
Cell. 1976 Apr;7(4):487-505 PMID: 986248
Prolonged exposure to acetylcholine: noise analysis and channel inactivation in cat tenuissimus muscle.
J Physiol. 1981 Jan;310:37-56 PMID: 6262505
Single-channel currents recorded from membrane of denervated frog muscle fibres.
Nature. 1976 Apr 29;260(5554):799-802 PMID: 1083489
Voltage-dependence of drug-induced conductance in frog neuromuscular junction.
Proc Natl Acad Sci U S A. 1975 Jun;72(6):2140-4 PMID: 1079601
A calcium-dependent transient outward current in Xenopus laevis oocytes.
Proc R Soc Lond B Biol Sci. 1982 Jul 22;215(1201):491-7 PMID: 6127718
The acetylcholine receptor: an "allosteric" membrane protein.
Harvey Lect. 1979-1980;75:85-254 PMID: 400609
The statistical nature of the acetycholine potential and its molecular components.
J Physiol. 1972 Aug;224(3):665-99 PMID: 5071933
Relaxation experiments using bath-applied suberyldicholine.
J Physiol. 1977 Jun;268(2):271-89 PMID: 301569
Noise analysis and channels at the postsynaptic membrane of skeletal muscle.
Prog Drug Res. 1980;24:9-56 PMID: 6256817
Acetylcholine receptors in the oocyte membrane.
Nature. 1977 Dec 22-29;270(5639):739-41 PMID: 22819
Cholinergic and catecholaminergic receptors in the Xenopus oocyte membrane.
J Physiol. 1982 Jul;328:143-70 PMID: 7131311
Active multi-subunit ACh receptor assembled by translation of heterologous mRNA in Xenopus oocytes.
Nature. 1981 Aug 27;292(5826):862-4 PMID: 7196502
Further observations on acetylcholine noise.
Nat New Biol. 1971 Jul 28;232(30):124-6 PMID: 4328026
Properties of the procaine end-plate potential.
Jpn J Physiol. 1957 Sep 30;7(3):199-212 PMID: 13491243
Voltage dependence of agonist effectiveness at the frog neuromuscular junction: resolution of a paradox.
J Physiol. 1975 Oct;251(2):245-70 PMID: 1081139

Article Info

Journal

The EMBO journal

Abbr.

EMBO J

ISSN

0261-4189

Published

1982-00-00

Pages

1307-12

Language

English

Region

England

NLM ID

8208664

PMCID

PMC553209

Subset

External Links

PubMed source PMC Full Text View journal details

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Properties of acetylcholine receptors translated by cat muscle mRNA in Xenopus oocytes.

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