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PMID: 6144664 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Identification of a glutaminyl-tRNA synthetase mutation Saccharomyces cerevisiae.

Journal of bacteriology ·Vol. 158 ·No. 2 ·1984-05-00 ·Pages 530-4

Mitchell AP, Ludmerer SW

Abstract

Saccharomyces cerevisiae glutaminyl-tRNA synthetase mutants were isolated through systematic screening of tight Gln- derivatives of a leaky glutamine auxotroph. These mutations define a single nuclear gene, GLN4. The gln4-1 mutation is specific for Gln-tRNA synthetase and shows a dosage effect in heterozygous diploids. The wild-type Gln-tRNA synthetase exhibits a Km for glutamine of 25 microM; the gln4-1 mutation increases this value 20-fold. These observations strongly suggest that GLN4 encodes the Gln-tRNA synthetase.

MeSH Terms
Amino Acyl-tRNA Synthetases/genetics,metabolism Genes Genes, Fungal Glutamates/metabolism Glutamic Acid Glutamine/metabolism Kinetics Mutation Saccharomyces cerevisiae/enzymology,genetics
Chemicals
Glutamates Glutamine Glutamic Acid Amino Acyl-tRNA Synthetases glutaminyl-tRNA synthetase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Mitchell A P
Ludmerer S W
References (9)
9 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.158.2.530-534.1984
Published
1984-05-00
Pages
530-4
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC215461
Subset
IM
Grants
NIADDK NIH HHS · AM13894 · United States
NIGMS NIH HHS · GM07446 · United States
NIGMS NIH HHS · GM15539 · United States
External Links
PubMed source DOI: 10.1128/jb.158.2.530-534.1984 PMC Full Text View journal details
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