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PMID: 6158050 Published · ppublish English Journal Article

Predicting coding function from nucleotide sequence or survival of "fitness" of tRNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 6 ·1980-06-00 ·Pages 3539-43

Pieczenik G

Abstract

The sequence of a nucleotide region of f1 bacteriophage was determined on a bonded ultrathin acrylamide gel with a discontinuous buffer system by using the dideoxy-DNA sequencing method. This sequence and one other were analyzed for maximal base pairing with tRNAs. The results allow a prediction of the direction and phase of possible coding functions. The implication of sequence constraints on mRNA codon frequency, tRNA structure, the origin of protein synthesis, and triplet reading are discussed in terms of neutral, Darwinian, and genotypic selectionist perspectives of evolution. The model of F. H. C. Crick, S. Brenner, A. Klug, and G. Pieczenik [(1976) Origins of Life 7, 389-397] for the origin of the genetic code is used to interpet contemporary adaptive and functional nucleic acid sequences.

MeSH Terms
Anticodon Base Sequence Codon Coliphages/genetics Genetic Code Models, Genetic RNA, Bacterial/analysis RNA, Transfer/analysis
Chemicals
Anticodon Codon RNA, Bacterial RNA, Transfer
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Pieczenik G
References (23)
23 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.77.6.3539
Published
1980-06-00
Pages
3539-43
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC349652
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.77.6.3539 PMC Full Text View journal details
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