Convenient assay for interferons.

Journal of virology ·Vol. 37 ·No. 2 ·1981-02-00 ·Pages 755-8

Rubinstein S, Familletti PC, Pestka S

Abstract

A convenient assay for interferons based on reduction of cytopathic effect was developed. The number of manipulations and the lengths of the various incubation steps were reduced to a minimum. The assay is simple to perform and can be completed within 16 h. Moreover, it can be used with various types of cells and a variety of viruses.

MeSH Terms

Animals Biological Assay/methods Cattle Cell Line Cytopathogenic Effect, Viral Fibroblasts Humans Interferons/analysis Kidney Vesicular stomatitis Indiana virus/growth & development

Chemicals

Interferons

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Rubinstein S

Familletti P C

Pestka S

References (9)

9 references, click to expand

Semi-micro, dye-binding assay for rabbit interferon.
Appl Microbiol. 1971 Apr;21(4):723-5 PMID: 4325022
Quantitative aspects of interferon-induced plaque reduction: kinetics of interferon action.
Virology. 1972 May;48(2):425-32 PMID: 4337029
Stabilization of interferon messenger RNA activity by treatment of cells with metabolic inhibitors and lowering of the incubation temperature.
Proc Natl Acad Sci U S A. 1973 Dec;70(12):3909-13 PMID: 4544057
The neuraminidase yield-reduction bioassay of human other interferons.
Proc Soc Exp Biol Med. 1975 Jun;149(2):433-8 PMID: 50598
Vesicular stomatitis virus plaque production in monolayer cultures with liquid overlay medium: description and adaptation to a one-day, human interferon-plaque.
J Clin Microbiol. 1976 Dec;4(6):479-85 PMID: 187619
Human leukocyte interferon: production, purification to homogeneity, and initial characterization.
Proc Natl Acad Sci U S A. 1979 Feb;76(2):640-4 PMID: 284389
Microassay for interferon, using [3H]uridine, microculture plates, and a multiple automated sample harvester.
Appl Environ Microbiol. 1980 Apr;39(4):823-7 PMID: 6155105
Established kidney cell lines of normal adult bovine and ovine origin.
Proc Soc Exp Biol Med. 1958 Jul;98(3):574-6 PMID: 13567776
Studies on vaccinia virus plaque formation and its inhibition by interferon. III. A simplified plaque inhibition assay of interferon.
Virology. 1963 Mar;19:302-9 PMID: 13930801

Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.37.2.755-758.1981

Published

1981-02-00

Pages

755-8

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC171063

Subset

External Links

PubMed source DOI: 10.1128/JVI.37.2.755-758.1981 PMC Full Text View journal details

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