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PMID: 6164523 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

In vitro RNA synthesis in oocyte nuclei of the newt Notophthalmus.

Chromosoma ·Vol. 82 ·No. 2 ·1981-00-00 ·Pages 171-87

Schultz LD, Kay BK, Gall JG

Abstract

An incubation medium is described which supports RNA synthesis in isolated oocyte nuclei of the newt Notophthalmus, and which permits subsequent autoradiographic examination of the lampbrush chromosomes and nucleoli. By using different concentrations of alpha-amanitin we distinguish RNA synthesis due to RNA polymerases I, II and III. All RNA synthesis on loops is inhibited by 0.5 microgram/ml of alpha-amanitin and is therefore due to polymerase II. Polymerase III is responsible for RNA synthesis at a small number of discrete sites in condensed chromatin. These include the centromere bars of three of four chromosomes, which probably represent 5S RNA synthesis, as well as 15-20 lesser sites scattered elsewhere. Polymerase I activity is confined to the nucleoli.

MeSH Terms
Amanitins/pharmacology Animals Autoradiography Cell Nucleolus/metabolism Cell-Free System Chromosomes/metabolism,ultrastructure DNA-Directed RNA Polymerases/metabolism Female Molecular Weight Oocytes RNA/biosynthesis Salamandridae Transcription, Genetic/drug effects
Chemicals
Amanitins RNA DNA-Directed RNA Polymerases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Schultz L D
Kay B K
Gall J G
References (33)
33 references, click to expand
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Article Info
Journal
Chromosoma
Abbr.
Chromosoma
ISSN
0009-5915
DOI
10.1007/BF00286102
Published
1981-00-00
Pages
171-87
Language
English
Region
Austria
NLM ID
2985138R
Subset
IM
Grants
NIGMS NIH HHS · GM12427 · United States
NICHD NIH HHS · HD 07180 · United States
External Links
PubMed source DOI: 10.1007/BF00286102 View journal details
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